CALLUS iNDUCTION, REGENERATION AND ACTIVE ) t . PRINCIPLE PRODUCTION FROM Urginea maritime (L.) BAKER | ||||
Journal of Plant Production | ||||
Article 14, Volume 31, Issue 12, December 2006, Page 7915-7935 PDF (241.59 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/jpp.2006.236431 | ||||
View on SCiNiTO | ||||
Authors | ||||
A. A. EI-Bakry,1; H. M. Soliman2; Heba S. Shahen3; Salta M. Ghazi1 | ||||
1Botany Dept Fac. of Sd., Heiwan University | ||||
2Pharmacognosy Dept., Fac. of Pharmacy, Helwan University | ||||
3Plant Blotechnològy Department, Genetic Engineering and Biotechnology Inst. Menofya University, Sadat City. | ||||
Abstract | ||||
The effect of different benzyl adenine (BA) concentrations and bulb explant zones on regeneration from Lirginea maritime bulbs was studied. Results showed that Z2 on BA conc. 4.0 mg/i gave the highest mean number of shoots after four months. The effect of different 2, 4aD concentrations and explant zones on the production of both somatic embryos and calli showed that callus production was highest on 2.0 mg/i 2,40 when using explants of Z2 and Z5.I-lighest frequency of embryo production on 0.25 mg/i 2,4-D, using Z4. The highest mean number of embryos was produced when explants of Z1 were treated with 4.0 mgll2, 4-D after 4 months. Using different naphthalene acetic acid (NAA) concentrations, Callus was produced with high frequency on 2.0 mg/i when Z2 explants were used and also on 4.0 mg/i when Zland Z4 explants were used. Mean number of embryos was highest on 1.0 mg/i NAA from ZI explants. Analysis of variance for the effect of NAA on explant zones showed significant differences between NAA concentrations, explant zones, and also for their interaction. Proscillaridin A (PsA) was purified from field collected material using silica gel columns and the structure was determined and identification carried out by means of El-MS, ‘HNMR and 13CeNMR. in vitro culture were initiated on different concentrations of BA and 2, 4-D. Both caIN and regenerated plants were tested for the presence of cardiac glycosides using thin layer chromatography (TLC) and quantified for PsA using HPLC. In calli PsA ranged from 0.10-0.1 8%DW in samples positive for PsA. Regenerated plants produced through organogenesis were positive for cardiac glycosides but negative for PsA except bulbs grown for 15 months from Z1 on media supplemented with 4.0 mg/I BA and 0.1 mg/i NAA which was 0.20% dry weight PsA. | ||||
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