The Ameliorative Potential of Alda-1 on Experimentally Induced Liver Fibrosis in Adult Male Mice. A Histological, Immunohistochemical and Biochemical Study | ||||
| Egyptian Journal of Histology | ||||
| Article 21, Volume 45, Issue 3, September 2022, Page 949-968 PDF (17.95 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejh.2022.133870.1674 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
amany solaiman   1; silvia Kamil Seddik Sawires2
| ||||
| 1histology and cell biology ,alexandria egypt | ||||
| 2histology and cell biology, alexandria university, Egypt | ||||
| Abstract | ||||
| Introduction: Because of its complex pathogenesis, liver fibrosis remains one of the diseases with no standard treatment. Alda-1 is considered as a promising drug in ameliorating such fibrosis. Aim of the Work: To evaluate the potential effect of Alda-1 after thioacetamide (TAA)-induced liver fibrosis in male mice. Materials and Methods: Twenty-five adult male mice (25–27 gm aged 2-3 months) were allocated into five equal groups. Group I (control group), group II (Alda-1 group): each mouse was given Alda-1 [5 mg/kg, intraperitoneally (ip)] twice weekly for four weeks. Group III (TAA group): each animal received TAA (200 mg/kg, ip) twice weekly for seven weeks. Group IV (TAA +Alda-1 group): each mouse was given TAA as group III. After stoppage of TAA administration, Alda-1 was given at a dose as group II and continued for four weeks. Group V (recovery group) each animal received TAA treatment as group III and left without any treatment for an extra four weeks. The histological changes were identified by the light (H&E and Masson’s trichrome stains) and electron microscopies, immunohistochemical and morphometric analysis. Blood samples were taken to evaluate the liver's function. Results: TAA caused marked histological and biochemical attenuation of hepatocytes structure and function with significant increase in collagen deposition. In addition, TAA caused significant elevation of liver enzymes. In Alda-1treated In Alda-1treated group (IV), hepatocytes revealed nearly normal structure, significantly decreased the elevated liver enzymes and significant increase in reduced glutathione and decrease in malondialdehyde in liver homogenate. Alda-1 decreased the elevated transforming growth factor, collagen-1 gene expression and the area percentage of collagen. In addition, alpha smooth muscle actin was significantly reduced. The anti-inflammatory effects were also detected by the decrease in the interleukin-6 and tumor necrosis factor-α. Conclusion: Alda-1 ameliorated TAA-induced liver fibrosis in mice. This might be due to its antioxidant, antifibrotic and anti-inflammatory effects. | ||||
| Keywords | ||||
| Alda-1; fibrosis; hepatocytes; histology; immunohistochemistry | ||||
|
Statistics Article View: 451 PDF Download: 512 |
||||
