EMBRYO CULTURE AS AN ALTERNATIVE WAY TO COMPLETE THE DEVELOPMENT OF EMBRYOS RESULTING FROM THE INTERSPESFIC CROSS BETWEEN A. odorum AND A. cepa | ||||
| Journal of Plant Production | ||||
| Article 11, Volume 30, Issue 2, February 2005, Page 891-896 PDF (67.51 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2005.237137 | ||||
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| Authors | ||||
| Naglaa A. Ashry1; H. E. Asfour2 | ||||
| 1Field Crops Res. inst., ARC, Giza, Egypt. | ||||
| 2Hort. Research Inst., ARC. Giza, Egypt. | ||||
| Abstract | ||||
| The widely spread onion belonging to Alium cepa L., is know to be severely affected by the soil born disease, white rot. Infected soils totally prevents the production of onion for a period up to ten years, which dramatically reduced the economic production of onion in Egypt and limited the exportation of Egyptian onion during the last decade. There were no source of resistance ¡n the cultivated onion. As an attemptation to pick out a source for resistance, a survey was made among other relatives belonging to Alium sp. Resistance was found in Alium odorum sp. and Alium tuberosum sp. Interspesific crosses were made for many years to transfer resistance ability to Egyptian onion cultivars, but normal crossing was always incomplete and embryos fail to develop normally. Tissue culture techniques were used to resque the embryos resulted from the cross between A. cepa and A. odorum. Embryos were collected and an array of media with different hormone combinations were examined. In this study a very rare percent of embryos succeeded to complete its development and produced plantlets. | ||||
| Keywords | ||||
| Tissue culture; Embryo culture; inter-specific cross; white rot; Alium sp | ||||
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