IMPACT OF METALLOTHIONINE2A GENE POLYMORPHISM ON THE LEVELS OF BLOOD HEAVY METALS AMONG WORKERS IN COPPER SMELTERS | ||||
| Egyptian Journal of Occupational Medicine | ||||
| Article 5, Volume 46, Issue 2, May 2022, Page 69-84 PDF (906.96 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejom.2022.237279 | ||||
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| Authors | ||||
| Mohammed RS* 1; Seif Eldin AS2; Khalifa EM1 | ||||
| 1Department of Occupational and Environmental Medicine, Faculty of Medicine, Cairo University | ||||
| 2Department of Occupational and Environmental Medicine, Faculty of Medicine, Cairo University, and Department of Health science, Faculty of Health and Rehabilitation Science, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia | ||||
| Abstract | ||||
| Introduction: Genetic variations are considered as internal contributors that play a vital role in heavy metals metabolism. Aim of Work: to evaluate the impact of metallothionine2A (MT2A) gene (rs10636 and rs28366003) single nucleotide polymorphisms (SNPs) on the levels of blood heavy metals among exposed workers in a copper smelters and detecting the genotypes associated with high levels of blood metals. Materials and Methods: A cross-sectional analytical study was conducted upon 65 exposed workers in a copper smelter at Helwan district and 65 non-exposed personnel as a control group. Both groups were subjected to a detailed questionnaire including age, duration of occupational exposure, smoking or other special habits, present, past and family histories. General, local examinations and laboratory investigations were done to determine blood levels of copper, lead and cadmium and to estimate genetic polymorphism of Metallothionine2A (MT2A) gene (rs10636 and rs28366003). Results: The GC genotype of rs10636 was the most prevalent genotype among the exposed group while the genotype GG was most common among nonexposed. As for rs28366003, we found GG was the most frequent genotype among the exposed group but AG was the most prevalent among non-exposed group. Blood levels of copper, lead and cadmium were highly elevated among the exposed group when compared to non exposed especially among those who carried GC genotype of rs10636 SNPs. As regards rs28366003, a significantly high level of copper was seen among subjects with GG genotype, but high levels of lead and cadmium were found with AA and AG genotypes respectively. Duration of exposure, GC genotype of rs10636 and GG of rs28366003 SNPs were significant predictors for high levels of copper, lead and cadmium. Conclusion: Metallothionine2A (MT2A) isoform gene polymorphism is associated with high levels of blood metals among exposed workers and it is considered as an important internal contributor that predispose to metal toxicity. | ||||
| Keywords | ||||
| Metallothionine2A (MT2A); Genetic polymorphism; rs28366003; rs10636; Heavy metals; Copper smelter | ||||
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