IDENTIFICATION AND SCREENING OF TOMPR02 eDNA IN SOME TOMATO GENOTYPES AND THE MORPHO· PHYSIOLOGICAL AND YIELD RESPONSE TO SAL T STRESS | ||||
| Journal of Plant Production | ||||
| Article 6, Volume 30, Issue 7, July 2005, Page 3707-3721 PDF (1.91 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2005.237414 | ||||
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| Authors | ||||
| Kh. A. EI-Halfawy,1; A. A. Guirgls1; Amal A. Abd EI-Aziz1; Suzan A. Swidan2; EI-S. A. El-Absawy1; W. S. EI-Sayed1 | ||||
| 1Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, Menofiya University, Sadat City. | ||||
| 2Horticulture Research Crops Institute, (ARC), Egypt. | ||||
| Abstract | ||||
| Four tomato genotypes were used in this investigation. They were the tolerant genotype of Lycopersicon penneflii, the seneitive genotype Lycopersicon escuiemum and two Egyptian tomato cultivars. Strain Band Floradid. They were exposed to four salt concentration (0.0, 4000, 8000 and 15000ppm) to identify the response of the morphological. physiological and yield traits. The biochemical and molecular analyses were also screened to identify tomPR02 gene. Plant height, plant fresh weight and root dry matter showed high response to salt, and the L. p < /em>enneflii was found to be the most tolerant genotype whereas, the wild genotype Lycopersicon esculentum seemed to be the most sensitive one. The proline content of the genotype Floradid showed the highest increment. The wild type showed the least increments in proline content. For yield response, L. penn/71i; showed the most tolerable. meanwhile wild type L. esculentum was the most seneitive one. Protein banding patterns screened after 3 days trealment, indicated the presence of a unique dark stained band only with genotype Strain B. After 33 days of treatment, a newly synthesized protein was observed under salt concentrations of 4000 and 8000 ppm. The cathodal Peroxidase isozyme band Ce at R.F. 0.36 cm was suggested to be a biochemical marker for salinity tolerance. A unique acid phosphatase isozyme band A1 was also observed at 4000ppm salt Cone. A full length (-2145pb) tomPR02 (P5CS) cDNA was amplified using RT-PCR from total RNA isolated from leaves of treated plants. The tomPR02 gene was also screened and amplified from different tomato genotypes to test their tolerance to salinity which was found to present in some and absent in others. These results might be of great help to the tomato breeders in Egypt, which enable them to chose the best and tolerant genotypes to be involved in their breeding programs. | ||||
| Keywords | ||||
| Acid phosphatase; Morphological responses. Peroxidase. Proline; RT- PCR; SOS·PAGE; tomPR02 gene; Salt stress; Tomato. and Yield response | ||||
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