TISSUE CULTURE ORGANOGENSISES IN SOYBEAN
Nasr,, M., EI-Halafawy, K., Hamdi, A., EI-Absawy, E., Zayed, E. (2005). TISSUE CULTURE ORGANOGENSISES IN SOYBEAN. EKB Journal Management System, 30(9), 5201-5208. doi: 10.21608/jpp.2005.237474
M. I. Nasr,; K. A. EI-Halafawy; A. Hamdi; E. EI-Absawy; E. M. Zayed. "TISSUE CULTURE ORGANOGENSISES IN SOYBEAN". EKB Journal Management System, 30, 9, 2005, 5201-5208. doi: 10.21608/jpp.2005.237474
Nasr,, M., EI-Halafawy, K., Hamdi, A., EI-Absawy, E., Zayed, E. (2005). 'TISSUE CULTURE ORGANOGENSISES IN SOYBEAN', EKB Journal Management System, 30(9), pp. 5201-5208. doi: 10.21608/jpp.2005.237474
Nasr,, M., EI-Halafawy, K., Hamdi, A., EI-Absawy, E., Zayed, E. TISSUE CULTURE ORGANOGENSISES IN SOYBEAN. EKB Journal Management System, 2005; 30(9): 5201-5208. doi: 10.21608/jpp.2005.237474

TISSUE CULTURE ORGANOGENSISES IN SOYBEAN

Journal of Plant Production
Article 10, Volume 30, Issue 9, September 2005, Page 5201-5208  XML PDF (117.61 K)
Document Type: Original Article
DOI: 10.21608/jpp.2005.237474
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Authors
M. I. Nasr,1; K. A. EI-Halafawy1; A. Hamdi2; E. EI-Absawy3; E. M. Zayed2
1Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, Minufiya University, Egypt.
2Cell Research Department, Field Crops Research Institute, Agricultural Research Center (ARC), Giza, Egypt.
3Bioinformatics Department, Genetic Engineering and Biotechnology Research Institute, Minufiya University; Egypt.
Abstract
The aim of this study was to initiate and maintain callus organogenesis cultures
of soybean and evaluate the response 01 seven soybean genotypes in callus
formation. The genotypes were selected on the basis of their reaction to cotton leaf
worm infection. This investigation was conducted in the field at Giza research station
in 2003 and 2004seasons. Immature embryos (INith 0.5-10 mm long) were excised
from immature seeds and used as ex-plant. The basal Murashige and Skoog (MS)
medium with addition of 4 times of micronutrients, Proline (1381 mgll), NAA (0.0372
mg/l) and SAP (2.996 mg/l) gave complete satisfaction for callus production.
Formation of shoots and roots were succeeded and rooted plantlets were
transplanted to pots in green house. The results indicted that the genotype L86K-73
was the best in response to tissue culture technique in soybean. Callus growth rate
was positively and significantly correlaled with each of number 01 shoots/callus and
number of roots. Therefore, both characters could be used to predict succeeding of
callus groYJth in soybean.
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