IN VITRO GENETIC ENHANCEMENT THE RESISTANT OF THREE GENOTYPES OF Zea mays L~ TO Fusarium oxysporum Phytotoxicity | ||||
| Journal of Plant Production | ||||
| Article 10, Volume 30, Issue 10, October 2005, Page 5919-5926 PDF (2.43 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2005.237515 | ||||
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| Author | ||||
| A. A. Haggran, | ||||
| Microbial Genetics Dept., National Research Center, Dokki, Cairo, Egypt | ||||
| Abstract | ||||
| The present investigation aimed to increase the tolerance in tissue culture of three hybrids (hybrid 151 , hybrid 158 and hybrid 352) of yellow maize (Zea maysL.) against the fungal pathogen (Fusarium oxysporum) through in vitro mutation induction via the chemical mutagen EMS in mature embryos. The induced calli after treated with EMS were selected and tested for their resistant activity against F. oxysporum. Then chitinase activity and protein fingerprint of the tested calli were detected. 0.6% EMS showed highly reduction in callus induction of the tested yellow mays (Zea maize L.) hybrids than the other EMS doses (0.4%) or the control. Control showed healthy calli ranged from 20 % to 40 % when inoculated with F. oxysporum. All treated calli with EMS showed increasing in healthy calli than the controls reached to 250, 150 and 170% of the hybrids; 151, 158 and 352, respectively when treated with 0.4% EMS. Also reached to 250, 167 and 267% of the hybrids; 151, 158 and 352, respectively when treated with 0.6% EMS. The mutagenized maize hybrids with EMS showed increasing in chitinase activity than their control treatments. Whereas the hybrids; 151, 158 and 352 reeched to 8.2§, 4.82 and 0.32%, respectively when treated by 0.4% EMS, the hybrids; 151, 158 and 352 reached to 30.40, 11.59 and 12.51%, respectively when treated with 0.6% EMS. The induced and inhibited bands from proteins could be used as genetic markers to detect the genetic response of different maize hybrids under EMS effect. | ||||
| Keywords | ||||
| Zea mays L; hybrids; tissue culture; mature embryo; mutation; EMS; F. oxysporum; chitinase and SOS-PAGE | ||||
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