IN VITRO PROPAGATION OF SOME FRUIT SPECIES. B- In vitro propagation of jojoba (Simmondsia chinensis Link, Schnider) plant. | ||||
| Journal of Plant Production | ||||
| Article 17, Volume 30, Issue 11, November 2005, Page 6983-6999 PDF (4.88 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2005.237757 | ||||
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| Authors | ||||
| M. A. Khamis,1; Wafaa T. Saeed2; A. H. Gad EI-Hak2 | ||||
| 1Hort. Dept. Faculty of Agric. Benha , Univ. | ||||
| 2Olive and semi-arid zone fruits Dept., Hort. Res. Institute, Agric. Res. Center, Cairo, Egypt. | ||||
| Abstract | ||||
| Vegetative propagation of jojoba plant is difficult by traditional methods. A factorial experiment was conducted to develop a protocol for cloning jojoba through tissue culture technique .In this concern, shoot tips and nodal cuttings were prepared from jojoba plants. After sterilization, the explant were initiated on three culture media i.e, MS , S5 and WPM each at either full, one half or one fourth strength, these media supplemented with 0.1 mg/L ISA, 1.0 mglL SA for establishment stage. After four weeks, MS medium gave the best results with the three measurements (survival %, shoot length and number of leaflets) full strength media proved to be the more suitable for the three measurements. Shoot tips surpassed nodal cutting explant. On the other hand, nodal cuttings which cultivated in quarter WPM had the lowest value in this respect. The newly formed shoots were transferred to the same media supplemented with either SA; Kinetin or 2ip at the concentration of 2, 4, 6 mglL for each through proliferation stage. Full strength MS medium supplemented with 2 mglL SA was superior and had the greatest number of shoots during the three subcultures. While the reverse was true with kinetin at 6 mg/L added to full strength WPM. Micro- shoots were rooted in the same half strength media with or without activated charcoal supplemented with 7 mg/L ISA + 1 mglL NAA plus either 1 or 1.5 mg/L caffeic acid. The data revealed that WPM was most suitable for the three rooting growth measurements (rooting perc:mtage, number of roots/plantlet and average root length) the presence of activated charcoal increased significantly the three rooting growth measurements iBA at (7 mglL) + NAA at (1mg/L) + caffeic acid at (1 mg/L) gave the highest value of rooting measurements . While, the reverse was detected by the charcoal omitted S5 medium supplemented with ISA at (7 mg/L) + NAA at (1 mglL) ~ caffeic acid at (1.5 mglL) during the two seasons of study. The plantlet produced from the best treatments of each medium, during the rooting stage were transplanted to (300 ml) plastic pots containing autoclaved transplanting media ( vermiculite : peat moss: sand mixed by volume (1: 1: 1) and maintained in green house for four weeks to investigate their effect on survival % , plant height and number of leaves per plant during acclimatization stage .The obtained results could be summarized as follows :- rooted plantlet in Y:. strength WPM + ISA (7 mg/L) + (1.0 mg/L) NAA + (1 mg/L) caffeic acid + 1.0 mg/L activated charcoal gave the highest value of rooting growth measurements while the reverse was true with rooted plantlet in Y:. strength 85 + ISA (7.0 mg/L) + ( 1.0 mg/L) NAA + (1.0 mg/L) caffeic acid + 1.0 mg/L activated charcoal. | ||||
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