The Effects of Melatonin on Caspase-3 and Antioxidant Enzymes Activity in Rats Exposed to Anticancer Drug
Yousif, S., Taqa, G., Taha, A. (2023). The Effects of Melatonin on Caspase-3 and Antioxidant Enzymes Activity in Rats Exposed to Anticancer Drug. EKB Journal Management System, 66(2), 283-289. doi: 10.21608/ejchem.2022.132312.5840
Salwan Yousif; Ghada Taqa; Ameer Taha. "The Effects of Melatonin on Caspase-3 and Antioxidant Enzymes Activity in Rats Exposed to Anticancer Drug". EKB Journal Management System, 66, 2, 2023, 283-289. doi: 10.21608/ejchem.2022.132312.5840
Yousif, S., Taqa, G., Taha, A. (2023). 'The Effects of Melatonin on Caspase-3 and Antioxidant Enzymes Activity in Rats Exposed to Anticancer Drug', EKB Journal Management System, 66(2), pp. 283-289. doi: 10.21608/ejchem.2022.132312.5840
Yousif, S., Taqa, G., Taha, A. The Effects of Melatonin on Caspase-3 and Antioxidant Enzymes Activity in Rats Exposed to Anticancer Drug. EKB Journal Management System, 2023; 66(2): 283-289. doi: 10.21608/ejchem.2022.132312.5840

The Effects of Melatonin on Caspase-3 and Antioxidant Enzymes Activity in Rats Exposed to Anticancer Drug

Egyptian Journal of Chemistry
Volume 66, Issue 2, February 2023, Page 283-289  XML PDF (1.18 MB)
Document Type: Original Article
DOI: 10.21608/ejchem.2022.132312.5840
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Authors
Salwan Yousif email 1; Ghada Taqaorcid 2; Ameer Taha3
1Department of Dental Basic Sciences, College of Dentistry. University of Mosul , Mosul , Iraq.
2Department of Dental Basic Sciences, College of Dentistry. University of Mosul , Mosul , Iraq
3Department of Biology, College of Education for Pure Science, University of Mosul , Mosul , Iraq
Abstract
This study was aimed to investigate the effects of Melatonin at )10mg/kg( on programmed cell death (apoptosis)by measurements Caspase -3 activity and antioxidant enzymes GSH, CAT, MDA, Peroxinitrate activity on rats exposed to anticancer drugs Gemcitabine. Sixty four adult male albino rats were used in this study ,weighing (250-350)gm with temperature 22±20C , The animals were feeding and drinking water ad libitum . the animals were divided into 5 main groups as following :Group A( Control group n=8 ) given a daily D.W. Group B:(melatonin group n=8)given a daily melatonin 10mg/kg/orally. Group C (Gemcitabine n=16)this group was divided into two group according to Gemcitabine dose (25 and 50 mg/kg).Group D (protective group n=16): this group was divided into two group according to Gemcitabine dose (25 and 50 mg/kg ) together with given a daily melatonin in dose (10mg /kg) orally. Group E (protective and treatment group n=16) : this group was divided into two group according to Gemcitabine dose (25 and 50 mg/kg ). This group was given a daily melatonin in dose (10mg /kg) orallyas a treatment. The results showed that melatonin reduced the activity of Caspase-3 enzyme (Apoptosis marker), MDA and peroxynitrate , that induced by chemotherapy drug ,While increased the activity of antioxidant enzyme GSH and CAT. Melatonin given protective effect on liver and kidney tissues from damaged that happened by Gemcitabine at two different dose (25,50 mg/kg ).
Keywords
Caspase -3; anticancer drugs; melatonin Antioxidant enzyme
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