Electrochemical sensor based on poly(methylene blue) with zinc oxide nanoparticles on glassy carbon electrodes for quantitative determination of vitamin B12
Abd-Alazize, N., Mohammedtayeb, A. (2023). Electrochemical sensor based on poly(methylene blue) with zinc oxide nanoparticles on glassy carbon electrodes for quantitative determination of vitamin B12. EKB Journal Management System, 66(2), 375-384. doi: 10.21608/ejchem.2022.135463.5962
Neam Abd-Alazize; Alaa Mohammedtayeb. "Electrochemical sensor based on poly(methylene blue) with zinc oxide nanoparticles on glassy carbon electrodes for quantitative determination of vitamin B12". EKB Journal Management System, 66, 2, 2023, 375-384. doi: 10.21608/ejchem.2022.135463.5962
Abd-Alazize, N., Mohammedtayeb, A. (2023). 'Electrochemical sensor based on poly(methylene blue) with zinc oxide nanoparticles on glassy carbon electrodes for quantitative determination of vitamin B12', EKB Journal Management System, 66(2), pp. 375-384. doi: 10.21608/ejchem.2022.135463.5962
Abd-Alazize, N., Mohammedtayeb, A. Electrochemical sensor based on poly(methylene blue) with zinc oxide nanoparticles on glassy carbon electrodes for quantitative determination of vitamin B12. EKB Journal Management System, 2023; 66(2): 375-384. doi: 10.21608/ejchem.2022.135463.5962

Electrochemical sensor based on poly(methylene blue) with zinc oxide nanoparticles on glassy carbon electrodes for quantitative determination of vitamin B12

Egyptian Journal of Chemistry
Volume 66, Issue 2, February 2023, Page 375-384  XML PDF (1.18 MB)
Document Type: Original Article
DOI: 10.21608/ejchem.2022.135463.5962
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Authors
Neam Abd-Alazize email 1; Alaa Mohammedtayeborcid 2
1College of Science, University of Mosul, Department of Chemistry, Iraq
2department of chemistry, college of science, university of Mosul, Mosul. Iraq
Abstract
Vitamin B12 is classified as being amongst the hydrophilic vitamins, and plays a crucial role in mortal physiology, and hemoglobin formation and function. It also promotes anti-inflammatory action and moderates risk from viral infection. This paper established an electroanalysis method to quantify vitamin B12 in commercially available supplements by designing a sensor using methylene blue and zinc oxide nanoparticles on a glassy carbon electrode (PMb/ZnO NPs/GCE) via cyclic voltammetry. This offers a wide linear range with high sensitivity for vitamin B12 detection via differential pulse voltammetry using the Co(II/I) redox pair. Sensor morphology and thickness were studied, as was the pH of the supporting electrolyte. To understand the influence co-species, an interference study was also conducted. Under optimized conditions, the redox peak current for Co(II/I) pair at -0.8 V vs. Ag/AgCl, with the linear relationship Ip = 0.0673x + 0.3449, r = 0.9942, showed a linear quantification range for vitamin B12 concentrations of 0.099–69.51 μM. The limit of detection was 0.0104 µM. Repeatability, sensitivity, and stability were evaluated. The PMb/ZnO NPs/GCE electrode developed was successfully applied to the determination of vitamin B12 in commercially available supplements. The recoveries obtained were in the range 97.1-104% for the injection and 95.9-103.3% for the tablet. The results obtained herein compared favorably with those from the current standard quantification via UV–vis spectrometry.
Keywords
poly(methylene blue); zinc oxide nanoparticles; vitamin B12
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