BIOLOGICAL ACTiVITIES OF DIFFERENT EXTRACTS AND ESSENTIAL OIL FROM GUAVA (Psidium guajava Linn) LEAVES GORWING IN EGYPT | ||||
| Journal of Soil Sciences and Agricultural Engineering | ||||
| Article 7, Volume 30, Issue 10, October 2005, Page 6421-6441 PDF (6.39 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jssae.2005.238249 | ||||
 View on SCiNiTO | ||||
| Author | ||||
| M. I. Kobeasy | ||||
| Biochemistry Dept., Fac. of Agric, Cairo Univ., Giza, Egypt. | ||||
| Abstract | ||||
| Phytochemical investigation of different extracts from guava (Psidium guajava L.) leaves revealed the presence of carbohydrates and/or glycosides, tannins, saponinis, f1avonoids, sterols and/or triterpenes and phenolic compounds while alkaloids and coumarins were absent. Also quantitative analysis of different extracts showed that crude ethanolic extract Et.1 contained more tannins, saponons, flavonoids and phenolic compounds (9.60%, 1.12%, 0.88% and 3.03%) than other extracts, also ethylacetate extract contained flavonoids and phenolic compounds (0.74% and 0.82%), respectively but aqueous extract contained moderate contents from these constituents (4.02%,1.32%,0.13% and 1.04%), respectively. Essential oil of dried P. guajava L. leaves was extracted by steam distillation and analysed by Gas chromatography Mass Spectroscopy (GC-MS). Essential oil yield was 0.67 g/100g from dried leaves. Thirty two components were identified by GC-MS and the major constituents were limonene (15.22%), Junipene (9.58%), glaucine (9.03%), beta terpinyl acetate (8.70%) and D-nerolidol (8.58%). The inhibitory effects of successive extraction of guava leaves with petroleum ether (PE), chloroform (Ch1), ethyl acetate (EA), ethanol 96% (Et.2), v-ater (W), complete ethanol 70% (Et.1) separately and three concentrations of essential oil on lipid peroxidation induced by Fe++/ascorbate in rat liver mitochondria and microsomes were determined. Also free radical scavenging activity of different extracts and essential oil were determined using 2,2 - diphenyl - 1 - picrylhydryzyl (DPPH·). The obtained results revealed that crude ethanolic extract Et.1 has high effect in protected rat liver mitochondria and microsomes from lipid peroxidation and was substantially more powerful antioxidant where the percentage of inhibition of lipid peroxidation in mitochondria and microsomes were (95.80% and 97.88% compared with rutin as standars (97.23% and 98.90%) and free radical scavenging using DPPH· was (94.38% and rutin 95.69%). Et.1 and essential oil were evaluated for the protective effect against liver damage induced by carbon tetrachloride (CCI4) in male albino rats. Rats were orally administered with 50 and 100 mg/kg body weight from ethanolic extract (Et.1) and essential oil for 14 days before CCl4 challenge and 100 mg from Et.1 and essential oil for toxicity analysis without CCl4 administration significantly damaged the liver as evident from very high activity of serum marker enzymes and glutathione - S - transferase. Also decrease total protein, albumin but increa-ed bilirubin contents in the serum. Et.1 administration significantly restored the elevated activities of Ii'ler marker enzymes, increased total protein and albumin, content enhanced the antioxidant enzyme activity also decrease bilirubin content than essential oil which gave slight effect at the same time Et.1 and essential oil at 100 mg/kg body weight had no any toxic effect. The results of antitumor activity of Et.1 and essential oil on Erhlisch ascites carcinoma (EACC) cells indicated that essential oil had higher antitumor activity than Et.1 which the dead cells percentage were ranged from 87 - 100% but in Et.1 ranged from 76 - 95%. | ||||
| Keywords | ||||
| Guava; Psidium gUejava Unn; leaves; extracts; essential oil; chemical composition; lipid peroxidation; DPPH·; free radicals; antioxidants; CCI4; hepatoprotective; anticancer | ||||
|
Statistics Article View: 41 PDF Download: 148 |
||||
