A STUDY ON IN VITRO PROPAGATION OF PINEAPPLE (Ananas comosus L. MERR.)
EI Masry,, H., Abd Rabou, F. (2004). A STUDY ON IN VITRO PROPAGATION OF PINEAPPLE (Ananas comosus L. MERR.). EKB Journal Management System, 29(1), 371-389. doi: 10.21608/jpp.2004.238478
H. EI Masry,; Farida A. Abd Rabou. "A STUDY ON IN VITRO PROPAGATION OF PINEAPPLE (Ananas comosus L. MERR.)". EKB Journal Management System, 29, 1, 2004, 371-389. doi: 10.21608/jpp.2004.238478
EI Masry,, H., Abd Rabou, F. (2004). 'A STUDY ON IN VITRO PROPAGATION OF PINEAPPLE (Ananas comosus L. MERR.)', EKB Journal Management System, 29(1), pp. 371-389. doi: 10.21608/jpp.2004.238478
EI Masry,, H., Abd Rabou, F. A STUDY ON IN VITRO PROPAGATION OF PINEAPPLE (Ananas comosus L. MERR.). EKB Journal Management System, 2004; 29(1): 371-389. doi: 10.21608/jpp.2004.238478

A STUDY ON IN VITRO PROPAGATION OF PINEAPPLE (Ananas comosus L. MERR.)

Journal of Plant Production
Article 10, Volume 29, Issue 1, January 2004, Page 371-389  XML PDF (293.57 K)
Document Type: Original Article
DOI: 10.21608/jpp.2004.238478
View on SCiNiTO View on SCiNiTO
Authors
H. EI Masry,1; Farida A. Abd Rabou2
1Tropical And Sub Tropical Fruit Research Department
2Horticulture Research Institute Agriculture Research Center, Giza, Egypt.
Abstract
In several trails for surface sterilization by immersing the explants in
Noacl (1 .5.10.15 %) + (1.0 %) Hg CI2 for 10.20.30 minutes. the least
contaminated explants were obtained by immersing the explants in NaOcl
(15%) + (1.0%) HgCI2 for 30 minutes.During the establishment stage after 3
weeks the explants were cultured on liquid and solid MS media. full and half
strength supplemented with SAP at 0 . 1 ,2 • 3 mg/L and ISA at 0 . 0.5 , 1.0
mglL for other 6 weeks. The best multiplication media was full strength liquid
MS media supplemented with 1 mglL BAP + 1 mg/L IBA .In rooting stage.
shootlet were cultured on media containing 0.5 x strength MS salts. 3 mg/L
activated charcoal and combinations of ISA at (0 . 0.2 • 0.5 , 1.0 . 2.0 mglL)
and NAA at (0 • 0.2 , 0.5 , 1.0 mglL) • the rooted shootlets were obtained by
culturing on MS media supplemented with 1 mg/L NAA + 0.5 mg/L ISA . At
hardening stage of 3 months duration by using mixture of peatmoos : sand •
the mixture of 3 peatmoss : 1 sand gave the highest percentage of survivals
and leaf number.
The acclimatization lasted for 6 months then fertilization experiments
on 12m onths old explants were carried out using a mixture of ammonium
sulphate (20%) . super phosphate (15% ) and potassium sulphate (48%) in
ten treatments as follows: 2:1:2 . 5:1:5 . 6:1:6 , 2:1.5:2 . 5:1.5:5,6:1.5:6.
2:2:2.5:2:5.7:2:7 and 7:1:7 these mixtures were applied to each pots in four
doses (0.5 . 1.0 • 1.5 • 2.0 gm) every week during the six months. the highest
values of survival percentage. plant length. stem length • stem thickness.
number of leaves and leaves area were obtained at ratio of 5:1.5:5 and 7:2:7
using a dose of 1 gm I plant every week.
Statistics
Article View: 37
PDF Download: 141