GC-MS, COUPLED OXIDATION ~CAROTENE AND DPPH' RADICAL OF Sa/via fruticosa VOLATILE OIL | ||||
Journal of Soil Sciences and Agricultural Engineering | ||||
Article 5, Volume 30, Issue 11, November 2005, Page 7205-7213 PDF (2.54 MB) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/jssae.2005.238500 | ||||
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Authors | ||||
F. A. Benkhayal1; M. H. AI-Saadi2; A. H. AI-Saadi3; H. M. AI-Sanousi4 | ||||
1Dept. of Food Science and Technology, College of Agric., University of Omar EL- Mukhtar, Libya | ||||
2Faculty of Pharmacy, University of Omar EL- Mukhtar, Libya | ||||
3Dept. of Zoology, College of Sci., Univ. of Omar EL- Mukhtar, Libya | ||||
4Dept. of Botany, College of Sci., Univ. of Omar EL- Mukhtar, Libya | ||||
Abstract | ||||
The volatile oil of Sa/via fruticosa which was extracted from the green leaves using hydrodistillation had a yellow color/aromatic and its percentage was 0.7% . The oil was studded for its constitutes and antioxidant activity . GC-MS results showed the presence of twenty three component , ten of them were found in high (1,8-Cineole ,1 ,8-Cineol Isomer ,Camphor, Trans-p-Caryophyllene .o-Plnene ,p-Myrcene, Linalool . a-Terpineol «x-Terpineol isomer, aCamphene) . The volatile oil was separated on silica gel plates using Benzene: Ethyl acetate (94:6 v/v) as a mobile phase. The separated bonds were sprayed by solution of p-carotene and linoleic acid, in order to determinate its antioxidant activity . only the bands of RF (0.21,0.35,0.93) showed an antioxidant effect. p-carotene bleaching test was used to measure the antioxidant effect and to compare its activity with BHT . The results revealed that a clear antioxidant activity of the oil when compared to blank. In an attempt to understand the mechanism by which the oil act antioxidant , DPPH' free radical test was used . A weak antioxidant effect was obtained for the five increased concentration used in this study when compared to BHT at 25 and 50 ppm . this could be attributed to the fact that the oil contain compounds which act as antioxidant by different mechanisms rather than its donation of proton (H+) | ||||
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