PRODUCTION OF SPOROPHYTIC PLANTS FROM SPORES OF Nephrolepis exaltata IN VITRO. | ||||
| Journal of Plant Production | ||||
| Article 17, Volume 29, Issue 3, March 2004, Page 1457-1472 PDF (330.73 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2004.238591 | ||||
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| Author | ||||
| Omaima M. Abdel- Kafie, | ||||
| Veget. and Floriculture Dept., Fac. of Agric., Mansoura Univ. | ||||
| Abstract | ||||
| The experiments have been achieved to examine a method to obtain homogenization using gametophytes as well as studying the effect of different culture media composition on sporophyte formation of Nep < /em>hro/ep < /em>is exaltata. The statistical analysis of data revealed the following findings: Spores were germinated after two weeks at 1/2 strength MS medium supplemented with agar at 1.5 gIL. In the first experiment (multiplication of the gametophytes colonies), the highest significant values of fresh weight (16.19 g) and length (6.02 ern) were obtained after five months as a result of modifying 3/4 strength MS medium with 2 mglL kinetin + 0.1 mglL NAA. In the second experiment in these stages. the highest significant fresh weight of gametophytes colonies value (17.66 g) was obtained as a-result of modifying MS medium with 4 mglL SAP + 0.2 mglL IAA after three months from spores germination. In sporophytes formation stage, MS 1/2 strength medium supplemented with 3 gmlsucrose + 3 giL AC induced the highest significant gametophyte colonies diameter (5 cm) in most treatments. However, MS 1/4 strength supplemented with 15 g sucrose recorded the lowest diameter value to about 2.66 cm. As for the percentage of gametophytes colonies showing sporophytes, the combination of MS 1/2 strength medium supplemented with sucrose (15 giL) significantly increased percentage of gametophytes colonies showing sporophytes formation of about 88%, while gametophytes which were cultured on MS 1/4 strength medium supplemented with sucrose (30 giL) nearly failed induce sporophytes. In hardening off stage, the highest significant survival percentage of hardened young sporophytes (99.91) was obtained in case of using 2 peat + 2 loam + 1 sand by volume culture medium. The best vegetative growth was reported with 2 peat + 2 loam + 2 sand medium (24 6 cm height, 47.66 fronds and 6.20 g dry weight) per plant. Also the same medium significantly increased values of chlorophyll, total carbohydrates, and total indoles. It could be concluded through this research, a fast life cycle and high sporophyte production on Nephrolep < /em>is exaltata, homogenization of gametophytes can be considered to be an excellent method for propagation, yielding incomparable numbers of sporophytes relatively in short period of time. | ||||
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