DETECTION OF TOBACCO MOSAIC TOBAMO VIRUS (TMV) ON SAFFLOWER IN EL-MANSOURA - DAKAHLlA, EGYPT | ||||
| Journal of Plant Production | ||||
| Article 11, Volume 29, Issue 5, May 2004, Page 2521-2527 PDF (439.1 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2004.238636 | ||||
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| Author | ||||
| M. A. El-Mazaty, | ||||
| Plant Pathology Department, Faculty of Agrlc., Mansoura Univ., Egypt. | ||||
| Abstract | ||||
| Tobacco mosaic virus (TMV) was isolated from safflower cv. Giza 1 plants showing mosaic symptoms In the field of EI-Mansoura region, Dakahlia, Egypt. Identification of virus Isolate was based on symptoms developed on diagnostic hosts, host range, physical properties, serological techniQues, including precipitin, agglutination, ouchterlony double diffusion and Rocket immunoelectrophoresis assay by using the specific antiserum to TMVand electron microscopy. Safflower TMV Isolate Induced systemic mosaic symptoms on Nicotiana tabaccum tv. Turkish and chlorotic local lesion without systemic spread on Chenopodium amaranticolot and C. quno8. Safflower TMV isolate was inactivated at 8SoC - gooe with stood dilution at 10.5 _10-43, longevity In vitro more \han 4 months. Safflower TMV virus Isolate was not transmitted by Myzus persicae, but transmitted mechanically. Transmlssion electron microscopy (TEM) indicate that, negative stained partially purified virus particles were rigid rod shaped with 300 - 310 nm In length and 18 nm in width. Protein subunit molecular weight was '18000. AU serologicallests with sap containing the virus gave strong reaction with TMV antiserum. | ||||
| Keywords | ||||
| Safflower; tobacco mosaic virus (TMV); identification, serology; electron microscopy | ||||
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