GENETIC BEHAVIOR OF SOME SORGHUM GENOTYPES FOR SOME FUNGI DISEASE RESISTANCE | ||||
| Journal of Plant Production | ||||
| Article 4, Volume 29, Issue 6, June 2004, Page 2989-3005 PDF (2.17 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2004.238643 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| S. A. Omar,; Fareida M. EI-Saled; K. I. Zaki | ||||
| Desert Research Center, EI-Matariya, Cairo, Egypt. | ||||
| Abstract | ||||
| Three field experiments were carried out at Maryoul. Ras·Sudr and EL· Maghara Stations, Desert Research Center, 2003 growing season to study the genetic behavior of nine sorghum genctypes of the M,o generation for the severity and incidence of leaf spot disease as well as to compare the fresh and dry forage yield with the parental cunivar (Giza·1). Isozymes (polyphenol oxidase and peroxidase) banding patterns and RAPO·PCR markers were used to genetically identify the genotypes studied for disease resistance. The results obtained revealed that genotypes differ significantly for forage yield at the three locations. however, the genotypes M16, M48, M32 and M43 gave a significant trend for fresh and dry forage yield compared with the parent cultivar (Giza-t), On the other hand, the results showed that genotype M ·2 was the most resistant one for fungi disease severity if compare 10 the parent cullivar lGiza·1) and the other genotypes among Ihe three field experiments. Also. the results revealed that different gene expression levels in the banding patterns when isozymes, which related to disease severity. For RAPO·PCR analysis. six random arbitrary primers were used; the results showed that there are polygel'letic relationships between the studied genotypes. Generally, the biochemical and molecular genetic analysis used in the present study successfully distinguished the fungi disease resistance genotypes of Sorghum blcolor L. | ||||
| Keywords | ||||
| Sorghum genotypes; Mutations; Heritability; lsozymes; RAPO· PCR; Fungi disease | ||||
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