DISTRBUTION OF BIOSURFACTANT PRODUCING BACTERIA IN SOME AERAS OF EGYPT | ||||
| Journal of Plant Production | ||||
| Article 7, Volume 29, Issue 6, June 2004, Page 3219-3230 PDF (1.27 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2004.238647 | ||||
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| Author | ||||
| M. Z. Sedik, | ||||
| Microbiology Department, Faculty of Agriculture, Cairo University | ||||
| Abstract | ||||
| 6iosurfactants are a unique class of biological compounds that have been shown to have a variety of potential applications. However, little knowledge is known about the distribution of biosurfactant - producing bacteria in the environment. The goal of this study was to evaluate how surfactant-producing bacteria are found in contaminated and uncontaminated sites. A twelve soil samples represent sandy or clay soils and a contaminated or uncontaminated areas were collected from different sites of Egypt and plated on nutrient aqar medium. The 494 colonies were obtained and screened for biosurfactant production in mineral salts medium. A nine bacterial isolates were selected as a positive action on biosurfactant production. The nine isolates were purified and grouped which yielded 2 unique groups. In which consist of 2 strains and 4 species. In addition the surface tension results demonstrated thai isolates produced different surfactant surface tension ranged between 25.1 to 50.2mN/m. The potential production of biosurfactanl is assessed based on the development of a fermentative process with a strain of Pseudomonas aeruginos8 (PHM7) which was produced highly amount of biosurfactant with a high reduction in surface tension. These production using different carbon sources (hexadecane, paraffinic oil, glycerol and olive oil) and nitrogen sources (NaN03, (NH.)2S04 and CH4N02) were tested. The more significant results were detected when glycerol was used as a carbon source, in amount of 2.0 gIL and NaN03 as a nitrogen source. | ||||
| Keywords | ||||
| Biosurfactanl; Soil; Bacteria; 8acillus; Pseudomonas | ||||
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