BEHAVIOUR OF EXPLANTS AND PLANTLETS OF DATE PALM UNDER IN VITRO AND IN VIVO SALINE CONDITIONS | ||||
| Journal of Plant Production | ||||
| Article 18, Volume 29, Issue 6, June 2004, Page 3495-3514 PDF (3.35 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2004.238668 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Hemat M. Kamal,1; M. H. Abd El-Zaher1; A. S. AI Adawe2 | ||||
| 1Pomorogy Dep.,Fac. Agric, Cairo Univ . | ||||
| 2Agricultural Research Institute. | ||||
| Abstract | ||||
| In vitro and in VIVO studies and proline analysis were carried out during 2002- 2003 seasons on two Egyptian cultivars of date palm (Zaghloul and Samani). The aims of these studies were tested the tolerant to in vitro and in vivo saline conditions in the two cultivars. Also.study the relationship between the proline content and the tolerant to saline conditions in these cunivars. Data revealed that all treatments of mercuric chloride gave the lowest contamination percentages (0.0% in both cultivars) whilst the treatment of Clorox al 10% for 15 min, gave the highest contamination percentages (70% in Zaghloul and 80% in Samani). The lowest survival percentages in both cultivars were obtained by the treatment of mercuric chloride at 0.1% for 10 min. The treatment of Clorox allO% for 15 min. gave the highest survival percentage in borh cullivars (100% in Zaghloul and Samani). while the treatment of mercuric chloride at 0.1% for 5 min. gave the highest survival disinfected explant percentage (60% in Zaghloul and 70% in Samani). Zaghloul cultivar was superior to Samani cultivar in averages of weight and diameter of callus; number and lenglh of the roots from in vitro shoots and the growth parameters in the acclimatization stage. On the other hend.Samani cultivar was superior to Zaghloul cultivar in averages of number and length ot in vitro shoots and growth parameters of in vitro embryos. The treatment of 7000 p.p.m. salts gave the lowest weight and diameter of the callus, the lowest number and length of in vitro shoots ; the lowest number and length of in vitro roots from in vitro shoots and the lowest growth of in vitro embryos compared with untreat. In vivo study showed that irrigation with solutions of 8000,9000 and 10000 ppm. salts gave the worest results (growth parameters) in the acclimatization stage for the two studied cultivars compared with the irrigalion with waler (control or untreat). There were reversely proportional between concentration of the salts and the growth parameters of the two studied cuftivars in either in vitro or in vivo conditions. Zagh/cul cultivar had more proline content than Saman! cultivar and exhibited more tolerant to either in vitro or in vivo saline conditions which reflected positive relationship between (he proline contents and the tolerant to saline conditions in the two cullivars. | ||||
|
Statistics Article View: 36 PDF Download: 129 |
||||
