INDIRECT CLONAL MICROPROPAGATION OF TWO YEMENI LOCAL GRAPE CUL TIVARS FROM SHOOT APICAL MERISTEMS IN VITRO | ||||
| Journal of Plant Production | ||||
| Article 20, Volume 29, Issue 10, October 2004, Page 5785-5790 PDF (1.8 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2004.238861 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| H. O. Elward,1; E. A. Hussein2; E. M. Almaidama1 | ||||
| 1Horticulture and Forests Department, Faculty of Agriculture, Sana'a University, Sana'a, Republic of Yemen. | ||||
| 2Biology Department, Faculty of Science, Sana'a University, Sana'a, Republic of Yemen. | ||||
| Abstract | ||||
| In the present study. trials were done in order to micropropagate two ocal and popular Yemeni grape Cultivars (Vitis vinifera L. cv. Asemi and cv. Razekl) by obtaining multiple adventitious shoot bud proliferation in vitro from shoot apical meristems using MS basal salts culture media containing different benzyl adenine and 2,4 0 concentrations. The experimental part of the study was carried out during 2002/2003 in the laboratories of the faculty of agriculture, Sana'a University. Results of the present study have revealed that multiple adventitious shoot bud formation was possible from the two selected cultivars under the effect of 1 or 2 mg/1. benzyl adenine plus 1 or 2 mg/1. of 2,40 within 4 to 6 weeks of incubation in the dark at room temperature. It was then possible to obtain regenerants from the adventitious shoot buds in culture tubes containing hormone free MS media. After acclimatization, the obtained regenerants were successfully transferred to soil. Results of analysis have shown that the obtained regenerants were identical to their corresponding controls morphologically but differed in their leaf chlorophyll contents. | ||||
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