LEAF CHARACTERISTICS AND DNA ANALYSIS OF IN OF VITRO REGENERATED PLANTLETS DATE PALM (Phoenix dactylifera, L.) | ||||
| Journal of Plant Production | ||||
| Article 10, Volume 29, Issue 12, December 2004, Page 6975-6991 PDF (3.82 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2004.238948 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| A. M. Abd EI-Hamid,; M. H. A. Abou-Bakr | ||||
| Dept. of Agric. Botany, Fac. of Agric., Cairo University, Giza, Egypt. | ||||
| Abstract | ||||
| The in vitro regenerated plantlets of date palm (Phoenix daetylifera L.) cv. Sewi, Via somatic embryogenesis, were visually screened at an early stage of their development; one foliage leaf. The observed off-types were classified into nine morphological groups and were compared to the normal plantlets with respect to morphological, anatomical characteristics of the leaf in addition to DNA profile using RAPD-PCR technique. The morphological variations included petiole shape and length, lamina shape, length, width and colour, whereas the main anatomical changes included mesophyll thickness, midrib zone, number of vascular bundles and xylem elements. DNA analysis revealed a high level of genetic variation correlated with the morphological changes. | ||||
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