Preparation and Purification of HBsAg from HBsAg-Positive Human Blood

Farag, Marwa Hany; Shouman, Sahar A.H.; El-Bayoumy, Ahmed S.A.; Abdel Mohty, Ahmed A.; Mehany, Nagy L.; Gado, Marwa M.

doi:10.21608/ejrsa.2022.128612.1133

	Preparation and Purification of HBsAg from HBsAg-Positive Human Blood
Egyptian Journal of Radiation Sciences and Applications
Volume 34, Issue 1, December 2021, Page 117-120 PDF (974.92 K)
Document Type: Original Article
DOI: 10.21608/ejrsa.2022.128612.1133
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Authors
Marwa Hany Farag ¹; Sahar A.H. Shouman²; Ahmed S.A. El-Bayoumy³; Ahmed A. Abdel Mohty⁴; Nagy L. Mehany³; Marwa M. Gado²
¹Labeled Compounds Department, Hot Laboratories Center, Atomic Energy Authority, Cairo, Egypt, [2] Microbiology Department, Faculty of Science, Ain Shams University, Cairo, Egypt,
²Microbiology Department, Faculty of Science, Ain Shams University, Cairo, Egypt
³Labeled Compounds Department, Hot Laboratories Center, Atomic Energy Authority, Cairo, Egypt
⁴Radioisotopes and Generator Department, Hot Laboratories Center, Atomic Energy Authority, Cairo, Egypt
Abstract
HEPATITIS B virus (HBV) is one of the most serious world health problems as it is highly distributed and can result in very dangerous diseases including liver cirrhosis, liver failure or even liver carcinomas. Thus, it is very important to early diagnose it. One of the most important tools to diagnose HBV is to measure the presence of hepatitis B surface antigen (HBsAg) in patient blood. A special kit is the one of the most potent tools for this purpose, in which HBsAg is essential component. Hence, in the present study the authors prepared and purified HBsAg which can be later used as an antigen, to prepare specific antibodies and for other applications.
Keywords
Hepatitis; Hepatitis B virus; Hepatitis B surface antigen; Production of antigen; Purification; Separation


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