IN VITRO PROPAGATION OF Gerbera jamesonii H. BOLUS | ||||
| Journal of Plant Production | ||||
| Article 29, Volume 29, Issue 12, December 2004, Page 7409-7422 PDF (3.69 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2004.238973 | ||||
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| Authors | ||||
| H. M. S. Aboshama; H. A. Emara | ||||
| Genetic Engineering and Biotechnology Research Institute. Minufiya University, Sadat City, Egypt. | ||||
| Abstract | ||||
| Shoot tips of Gerbera jamesonii H. Bolus were used as explants in this study. Aseptic cultures of Gerbera were established by placing the isolated shoot tips on .Murashige and skoog medium supplemented with different cytokinins (SA, Kin or 2ip) at different concentrations. The highest number of shoots/explant and the heaviest c fresh weight/culture were obtained when MS medium contained 2.0 mg/L SA. Leaves of the obtained aseptic cultures were examined as explants to produce shoots (direct organogenesis). Interestingly, while intact leaves showed positive response in shoot formations on the petioles, there were no shoot formations on cut leaves (without petioles). This study recommended using intact old leaves of gerbera as they showed higher response on shoot formations when compared with the response of young leaves. While, the highest number of shoots was obtained on the petiole when the intact old leaf was cultured on MS medium contained 3 mg/L BA, the highest percentage of old leaves, that produced shoots, was achieved with the medium -contained 1, 2 or 3 mg/L SA. While at rooting stage, the highest response of root formations was achieved when the medium supplemented with 0.5 mg/L ISA, in the same time proved to be the most effective in increasing the root lengths. The plantlets were successfully acclimatized when transferred to pots contained soil mixer of 'peatrnoss: sand : vermiculite in equal volumes (1: 1: 1). The survival percentage was 100% after one month from tans planting. Abbreviations: MS: Murashige and Skoog; 2,4-D : dichlorophenoxyacetic acid; SA: Senzyladenine; NAA: naphthalene acetic acid; IBA: indole-3-butyric acid; Kin: Kinetin; 2ip (2-isopenteyladenine . | ||||
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