EFFECT OF UV-C STRESS ON TOTAL PHENOLICS, TOTAL FLAVONOIDS AND KHELLIN CONTENTS IN AMMI VISNAGA (L.) LAM. FRUITS
Khallaf, I., Hussein, R., Ali, O., Remeih, G., Mortada, A., Mohamed, K. (2022). EFFECT OF UV-C STRESS ON TOTAL PHENOLICS, TOTAL FLAVONOIDS AND KHELLIN CONTENTS IN AMMI VISNAGA (L.) LAM. FRUITS. EKB Journal Management System, 45(1), 89-97. doi: 10.21608/bfsa.2022.239242
Iman S. A. Khallaf; Rania A. A. Hussein; Osama M. M. Ali; Gehad S. Remeih; Aya S. Mortada; Khaled M. Mohamed. "EFFECT OF UV-C STRESS ON TOTAL PHENOLICS, TOTAL FLAVONOIDS AND KHELLIN CONTENTS IN AMMI VISNAGA (L.) LAM. FRUITS". EKB Journal Management System, 45, 1, 2022, 89-97. doi: 10.21608/bfsa.2022.239242
Khallaf, I., Hussein, R., Ali, O., Remeih, G., Mortada, A., Mohamed, K. (2022). 'EFFECT OF UV-C STRESS ON TOTAL PHENOLICS, TOTAL FLAVONOIDS AND KHELLIN CONTENTS IN AMMI VISNAGA (L.) LAM. FRUITS', EKB Journal Management System, 45(1), pp. 89-97. doi: 10.21608/bfsa.2022.239242
Khallaf, I., Hussein, R., Ali, O., Remeih, G., Mortada, A., Mohamed, K. EFFECT OF UV-C STRESS ON TOTAL PHENOLICS, TOTAL FLAVONOIDS AND KHELLIN CONTENTS IN AMMI VISNAGA (L.) LAM. FRUITS. EKB Journal Management System, 2022; 45(1): 89-97. doi: 10.21608/bfsa.2022.239242

EFFECT OF UV-C STRESS ON TOTAL PHENOLICS, TOTAL FLAVONOIDS AND KHELLIN CONTENTS IN AMMI VISNAGA (L.) LAM. FRUITS

Bulletin of Pharmaceutical Sciences Assiut University
Article 8, Volume 45, Issue 1, June 2022, Page 89-97  XML PDF (630.32 K)
Document Type: Original Article
DOI: 10.21608/bfsa.2022.239242
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Authors
Iman S. A. Khallaf* 1; Rania A. A. Hussein2; Osama M. M. Ali2; Gehad S. Remeih2; Aya S. Mortada2; Khaled M. Mohamed3
1Pharmacognosy Department, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt
2Student at Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt
3Pharmacognosy Department, Faculty of Pharmacy, Fayoum University, Fayoum, 63514, Egypt
Abstract
Ammi visnaga fruits (L.) Lam., is widely used for its antispasmodic and vasodilating effects. These effects are attributed to its main content of khellin. In our research, we studied the effect of UV-C stress on the metabolite production of the fruits. Fruits were subjected to UV-C treatment for ½, 1, 2, 3, 4 and 5 hr The change in the metabolic profile was studied by the quantification of total phenolics, total flavonoids and khellin in the methanolic extracts of the fruits applied to stress and extracts of control fruits. Total phenolics and total flavonoids were quantified colorimetrically using appropriate reagent for each. Folin-Ciocalteau reagent was applied for the quantification of total phenolics while 2% AlCl3 solution was applied for the quantification of flavonoids.  On the other hand, khellin was quantified by HPLC analysis. Our results showed that the concentrations of total phenolics, total flavonoids and khellin contents of the fruit extracts are significantly increased by exposing the fruits to UV-C stress with maximum production at 1 and 2 hr.
Keywords
Ammi visnaga; Folin-Ciocalteau reagent; Khellin; UV-C stress; Total phenolics; Total flavonoids
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