Gas Chromatography-Mass Spectrometry Analysis of Phytocomponents Present in Pimpinella anisum L. Callus Cultures as Affected by Yeast and Phenylalanine Application
Arafa, N., Moawad, M., Aly, U. (2022). Gas Chromatography-Mass Spectrometry Analysis of Phytocomponents Present in Pimpinella anisum L. Callus Cultures as Affected by Yeast and Phenylalanine Application. EKB Journal Management System, 65(131), 667-675. doi: 10.21608/ejchem.2022.129687.5728
Nermeen M. Arafa; Mahmoud Moawad; Usama Aly. "Gas Chromatography-Mass Spectrometry Analysis of Phytocomponents Present in Pimpinella anisum L. Callus Cultures as Affected by Yeast and Phenylalanine Application". EKB Journal Management System, 65, 131, 2022, 667-675. doi: 10.21608/ejchem.2022.129687.5728
Arafa, N., Moawad, M., Aly, U. (2022). 'Gas Chromatography-Mass Spectrometry Analysis of Phytocomponents Present in Pimpinella anisum L. Callus Cultures as Affected by Yeast and Phenylalanine Application', EKB Journal Management System, 65(131), pp. 667-675. doi: 10.21608/ejchem.2022.129687.5728
Arafa, N., Moawad, M., Aly, U. Gas Chromatography-Mass Spectrometry Analysis of Phytocomponents Present in Pimpinella anisum L. Callus Cultures as Affected by Yeast and Phenylalanine Application. EKB Journal Management System, 2022; 65(131): 667-675. doi: 10.21608/ejchem.2022.129687.5728

Gas Chromatography-Mass Spectrometry Analysis of Phytocomponents Present in Pimpinella anisum L. Callus Cultures as Affected by Yeast and Phenylalanine Application

Egyptian Journal of Chemistry
Volume 65, Issue 131, December 2022, Page 667-675  XML PDF (310.18 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2022.129687.5728
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Authors
Nermeen M. Arafaorcid 1; Mahmoud Moawadorcid 2; Usama Aly email orcid 3
1National Research Center
2Department of Pathology, National Cancer Institute, Cairo University, Cairo, Egypt
3Plant Biotechnology, Biotechnology Research Institute, National Research Center
Abstract
Long times ago, anise (Pimpinella anisum L.) plant, which belonging to umbelliferae family, was used in Egyptian folk medicine. In the current study, the effects of yeast extract as an elicitor and phenylalanine as a precursor on the accumulation of bioactive constituents in anise callus cultures were documented by gas chromatography-mass spectrometry (GC-MS) techniques. It is revealed that P. anisum callus extracts contain different biologically active compounds with antioxidant, antimicrobial and anticancer potential. Cytotoxicity of anise callus extracts was studied to illuminate the vital assignment of many active constituents in diminution of the breast cancer growth and also their antitumor activity.
Keywords
Pimpinella anisum; Callus; GC-MS; Active constituents
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