FACILE HPLC/UV METHOD FOR DETERMINATION OF METFORMIN AND 1-CYANOGUANIDINE USING NOVEL HALOGENATED STATIONARY PHASE | ||||
| Bulletin of Pharmaceutical Sciences Assiut University | ||||
| Article 18, Volume 45, Issue 1, June 2022, Page 223-231 PDF (655.64 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bfsa.2022.239443 | ||||
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| Authors | ||||
Ali Abdel-Hakim* 1; Aliaa I. Shallan 2; Mohamed A. Hammad1; Maha M. Abou El- Alamin* 2
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| 1Analytical Chemistry Department, Faculty of Pharmacy, University of Sadat City, Sadat City, Egypt | ||||
| 2Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Helwan University, Cairo, Egypt | ||||
| Abstract | ||||
| Metformin is the most prescribed drug for diabetes. We aimed to develop a new HPLC method for determination of metformin and its main impurity 1-cyanoguanidine in raw material and its pharmaceutical tablets. The separation was achieved on pentabromobenzyl column using a mixture of 20% 10.0 mM phosphate buffer (pH 5.5) and 80% methanol as a mobile phase at a flow rate of 1.0 mL min-1 and UV detection at 227 nm. The analysis time was less than 4.0 minutes. The method was linear through concentration ranges of 3.0 -80 µg mL-1 and 0.5-10 µg mL-1 and detection limits of 0.73 µg mL-1 and 0.11 µg mL-1 for metformin and 1-cyanoguanidine, respectively. The method was successfully applied for determination of metformin in bulk and pharmaceutical dosage forms. The high sensitivity of the developed method allowed for determination of 1-cyanoguanidine below its specified limits in metformin raw material and tablets. | ||||
| Keywords | ||||
| metformin; cyanoguanidine; HPLC; pentabromobenzyl | ||||
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