EFFECTS OF MAGNETIC-ACTIVATED SPERM CELL SORTING TECHNIQUE IN BLASTOCYST FORMATION AFTER INTRACYTOPLASMIC SPERM INJECTION | ||||
| Scientific Journal for Damietta Faculty of Science | ||||
| Volume 11, Issue 2, December 2021, Page 10-21 PDF (831.41 K) | ||||
| Document Type: Original articles | ||||
| DOI: 10.21608/sjdfs.2021.239486 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
MAY RIFKEY ELZANATEY  1; Amoura Abou ElNaga2; Hekmat El-Gammal3; Mohamed El-Zayat4
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| 1ZOOLOGY Department ,faculty of Science,Damietta University , Damietta Egypt | ||||
| 2Faculty of Science, Mansoura University | ||||
| 3Zoology Department, Faculty of Science, Damietta University | ||||
| 4Royal Center | ||||
| Abstract | ||||
| Many sperm preparation procedures are used in intracytoplasmic sperm injection (ICSI), including migration, density gradient centrifugation, and filtering. Magnetic-activated cell sorting (MACS) is a type of magnetic separation that has been used in a variety of biomedical and biological studies. To examine the fertilisation rate, cleavage, and development of Blastocystes in two sperm preparation procedures for ICSI, MACS and density gradient centrifugation (DGC). The research was carried out in the Royal Center in Mansoura, Dakahlia Governorate, Egypt. Fifty patients receiving ICSI had their sperm samples taken. The sperm were separated into two groups using traditional DGC. One group served as DGC, while the other served as MACS. The number of oocytes harvested, the number of metaphase II oocytes injected, fertilisation rates, cleavage rates, and embryo quality were all compared. In terms of fertilisation rate and cleavage, there was a statistically significant difference between the MACS and DGC groups. In terms of high quality, there is a statistically significant difference between the two groups in terms of the quantity and rate of good quality embryo A and B. The number of MII oocytes and age had a statistically significant negative connection. Furthermore, in the DGC group, there was a statistically significant negative connection between sperm DNA fragmentation (SDF) and fertilisation rate. MACS sperm selection of human spermatozoa is a safe, straightforward, and effective procedure for sperm preparation in a clinical environment for ICSI. | ||||
| Keywords | ||||
| Intracytoplasmic sperm injection; sperm preparation; magnet activated cell sorting; density gradient centrifugation; blastocysts | ||||
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