PRODUCTION OF CELL WALL-HYDROLYZING ENZYMES AND GC-MS EXTRACT ANALYSIS OF BYSSOCHLAMYS LAGUNCULARIAE, A NEW RECORD ISOLATED FROM EGYPT
Al-Bedak, O., Abdel-Sater, M., Galal, H., Hassan, S. (2022). PRODUCTION OF CELL WALL-HYDROLYZING ENZYMES AND GC-MS EXTRACT ANALYSIS OF BYSSOCHLAMYS LAGUNCULARIAE, A NEW RECORD ISOLATED FROM EGYPT. EKB Journal Management System, 45(1), 359-369. doi: 10.21608/bfsa.2022.239545
Osama A. Al-Bedak; Mohamed A. Abdel-Sater; Hagar M.K. Galal; Sedky H.A. Hassan. "PRODUCTION OF CELL WALL-HYDROLYZING ENZYMES AND GC-MS EXTRACT ANALYSIS OF BYSSOCHLAMYS LAGUNCULARIAE, A NEW RECORD ISOLATED FROM EGYPT". EKB Journal Management System, 45, 1, 2022, 359-369. doi: 10.21608/bfsa.2022.239545
Al-Bedak, O., Abdel-Sater, M., Galal, H., Hassan, S. (2022). 'PRODUCTION OF CELL WALL-HYDROLYZING ENZYMES AND GC-MS EXTRACT ANALYSIS OF BYSSOCHLAMYS LAGUNCULARIAE, A NEW RECORD ISOLATED FROM EGYPT', EKB Journal Management System, 45(1), pp. 359-369. doi: 10.21608/bfsa.2022.239545
Al-Bedak, O., Abdel-Sater, M., Galal, H., Hassan, S. PRODUCTION OF CELL WALL-HYDROLYZING ENZYMES AND GC-MS EXTRACT ANALYSIS OF BYSSOCHLAMYS LAGUNCULARIAE, A NEW RECORD ISOLATED FROM EGYPT. EKB Journal Management System, 2022; 45(1): 359-369. doi: 10.21608/bfsa.2022.239545

PRODUCTION OF CELL WALL-HYDROLYZING ENZYMES AND GC-MS EXTRACT ANALYSIS OF BYSSOCHLAMYS LAGUNCULARIAE, A NEW RECORD ISOLATED FROM EGYPT

Bulletin of Pharmaceutical Sciences Assiut University
Article 30, Volume 45, Issue 1, June 2022, Page 359-369  XML PDF (1.05 MB)
Document Type: Original Article
DOI: 10.21608/bfsa.2022.239545
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Authors
Osama A. Al-Bedak1; Mohamed A. Abdel-Sater2; Hagar M.K. Galal3; Sedky H.A. Hassan email 4
1Assiut University Mycological Centre, Assiut University, Assiut 71511, Egypt
2Department of Botany and Microbiology, Faculty of Science, Assiut University, Assiut, Egypt
3Department of Botany and Microbiology, Faculty of Science, New Valley University, El-Kharga 72511, Egypt
4Department of Botany and Microbiology, Faculty of Science, New Valley University, El-Kharga 72511, Egypt/ Department of Biology, College of Science, Sultan Qaboos University, Muscat 123, Oman
Abstract
In the present work, six strains of Byssochlamys lagunculariae were isolated from alkaline wastewater of textile factory polluted with azo dyes in Alexandria, Egypt. Out of the six strains, only one was identified using the sequencing of the internal transcribed spacer gene (ITS). ITS sequence was uploaded to GenBank (MT939911) and a pure culture was maintained in the culture collection of Assiut University Mycological Centre (AUMC) as B.lagunculariae AUMC 14498. The strain shared some morphological characteristics indicating species such as the existence of smooth chlamydospores, ascospore size, and good growth on Czapek’s yeast autolysate agar (CYA) at 30 °C. However, it was different from the type species by providing larger conidia and positive acid production on creatine sucrose agar (CREA) at 30 °C. Extrolite analysis of this strain was performed using gas chromatography mass spectrometry (GC-MS). The extrolite analysis extract revealed that more than fifty chemical compounds were detected, which have an important medical application. All B. lagunculariae strains tested, when grown on oat spelt xylan could utilize oat spelt xylan to produce endoglucanase, exoglucanase and xylanase as well as maize starch and pectin, respectively for amylase and pectinase production. Three strains of B. lagunculariae achieved maximum specific activity for amylase ranged from 75.65 to 114.67 IU/mg, while one of these three was superior for endoglucanase (59.1 IU/mg), exoglucanase (102.84 IU/mg), pectinase (34.33 IU/mg), and xylanase (90.21 IU/mg) production.
Keywords
Byssochlamys lagunculariae; B. lagunculariae enzymes; B. lagunculariae extrolite; : Byssochlamys isolation
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