GENETIC IMPROVEMENT OF Bacillus thuringiensis AS A BIOCONTROL AGENT AGAINST BiomphaJaria aJexandrina SNAIL. | ||||
| Journal of Plant Protection and Pathology | ||||
| Article 7, Volume 29, Issue 9, September 2004, Page 5317-5334 PDF (23.9 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jppp.2004.240014 | ||||
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| Authors | ||||
| Tahany M. A. EI-Kawokgy,1; M. E. M. Zowail2; Wafaa K. Hegazy1; Halima H. Salem3 | ||||
| 1Microbial Genetics Dept., NRC, Dokki, Cairo, Egypt. | ||||
| 2Zoolgy Dept., Fac.of Sci., Zagazig Univ., Banha, Egypt. | ||||
| 3Nucleic acid Dept., Mubarak City for Sci.Res. and Techn. Applic. Alexandria, Egypt | ||||
| Abstract | ||||
| Susceptibility of Bacillus thuringiensis spores and toxin to UV-C (254nm) of the solar spectrum reaching earth's surface may be responsible for its inactivation and low persistence in nature. Spores of the Bt israeiensis (1977 &HD522) were more resistant to UV-C radiation than spores of at tenebrionfs (tt4) and the isolated strain (66). Maximum molluscicidal activities were obtained following successive rounds of exposing the 2 strains; tt4 and 66 to UV-C. The mutants obtained designated as 6615c & tt4/2a showed 100% mortality of Biomphalarla a/exandrina snails, while their parental strains showed 60% & 80% of mortality, respectively. The highly toxic mutants derived from at. (66 & tt4) appeared to produce number of crystals more than that of their parental strains. The protein pattems at these mutants indicated that they produced polypeptide of 65 KDa or its derivatives that caused high toxicity of these mutants. ,.. ~ DNA profile of some mutants was established by using polymerase chain reaction (PCR). 111e PCR screen can identify mutants with altered electrophoretic panerns containing novel genes. | ||||
| Keywords | ||||
| BaCillus thuringiensis; Mutation; Biomphalaria aiexandrina snail; UV- radiation | ||||
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