Animal Model of Non-Alcoholic Steatohepatitis in Histology Department Sohag University | ||||
| Sohag Medical Journal | ||||
| Article 2, Volume 26, Issue 2, May 2022, Page 12-18 PDF (1.1 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/smj.2022.125601.1306 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Samira M. Mohamed  1; Doha S. Mohammed2; Nesreen G. Abd El-Haliem2; Ahmed O. Ahmed 3; Eman E. Abu-Dief4
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| 1Histology Department, Faculty of Medicine, Sohag University | ||||
| 2Department of Histology, Faculty of Medicine, Sohag University, Egypt | ||||
| 3Internal Medicine, Faculty of Medicine , sohag university, sohag, egypt | ||||
| 4Histology department, Faculty of Medicine, Sohag University | ||||
| Abstract | ||||
| Non-alcoholic fatty liver disease (NAFLD) is characterized by the deposition of triglycerides in the hepatocytes causing their injury resulting in non-alcoholic steatohepatitis (NASH), fibrosis, and cirrhosis, as a healing response, and may progress to hepatocellular carcinoma (HCC). We aimed to establish an animal model for NAFLD/NASH by the use of a high-fat diet. A total number of 30 adult male mice were categorized into two groups; control, high-fat diet (HFD) 15 animals each. The livers of these animals were excised and processed for histological examination for the progress of NAFLD/NASH changes. At the end of the experiment, the mice were slaughtered at 11, 13, 15, 17-week intervals. Liver specimens and blood samples from all animal groups were collected. The liver specimens were processed and stained with hematoxylin & eosin. Blood samples were processed for liver functions. Our results demonstrate that NASH was established 17 weeks after high fat diet administration | ||||
| Keywords | ||||
| Animal Model; Non-Alcoholic Steatohepatitis; high-fat diet | ||||
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