Ameliorative activities of Moringa oleifera ethanolic leaf extract on Cadmium chloride-induced toxicity in the kidney of adult Wistar rats: A microscopic assessment | ||||
| Egyptian Journal of Histology | ||||
| Articles in Press, Accepted Manuscript, Available Online from 30 May 2022 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejh.2022.130028.1667 | ||||
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| Authors | ||||
Okikioluwa Aladeyelu1; Abdulfatai Olakunle Ojewale  2; Darlington Onyejike3; Collins Obi4; Oloruntoba Akintayo5; Joseph Arusiwon6
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| 1Discipline of Clinical Anatomy, Nelson R Mandela School of Medicine, University of Kwazulu-Natal, Durban, South Africa | ||||
| 2Department of Anatomy, Faculty of Basic Medical Sciences, Lagos State University College of Medicine, Ikeja, Lagos. | ||||
| 3Department of Anatomy, Faculty of Basic Medical Sciences, Nnamdi Azikiwe University, Nnewi Campus, Anambra, Nigeria | ||||
| 4Department of Medical Laboratory Science, Faculty of Health Science, University of Nigeria, Enugu Campus, Enugu, Nigeria | ||||
| 5Department of Physiology, College of Medicine and Health Sciences, Afe Babalola University, Ado Ekiti (ABUAD), Ekiti, Nigeria | ||||
| 6Department of Anatomy, Nnamedi Azikiwe University, College of Health Sciences, Nnewi, Nigeria | ||||
| Abstract | ||||
| Introduction: The exposure of humans to cadmium since its commercialization due to its agricultural and industrial application has adverse effects on body organs and tissues such as the kidney. Moringa oleifera was investigated for its ameliorative effect on cadmium chloride-induced toxicity on renal tissue. Materials and Methods: Forty-six (46) male Wistar rats weighing between 160 – 180 g were used for the study. Twenty-six rats were used to determine LD50 of cadmium and M. oleifera, while twenty (20) rats were randomly divided into 4 groups: A, B, C, and D (n= 5). Group A was the control group receiving only animal feed and distilled water. Group B was the cadmium group that received 50 mg/kg of cadmium chloride for 4 weeks. Group C and D were the treatment groups that received 50 mg/kg of cadmium chloride alongside ethanolic leaf extract of M. oleifera at different doses of 1000 mg/kg and 2000 mg/kg, respectively, for 4 weeks. At the end of the experiment, animals were sacrificed with diethyl ether, and the kidney was harvested and immediately fixed in 10% bouins fluid for histological assessment. Results: The result showed a normal kidney histo-architecture of animals in the control group. In the cadmium group, degeneration and necrosis of tubular cells with narrowing of podocytes and glomerular cells in the Bowman’s capsule were observed in the kidney of animals in this group. However, the animals in both treatment groups showed a non-distorted histo-architecture of the kidney. In group C, the renal tubular and glomerular cells were properly embedded in the Bowman’s capsule. In group D, the histo-architecture of the kidney appeared in the approximate status as the control group. Conclusion: This study concluded that the ethanolic leaf extract of M. oleifera showed appreciable potential in alleviating the toxic effect of cadmium on the kidney. | ||||
| Keywords | ||||
| Cadmium; Moringa oleifera; Kidney; Wistar rats; Microscopic | ||||
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