Processed Cheeses Fortified by Laurus Nobilis L. Extract Nanoemulsion Ameliorate Hyperhomocysteinemia in Ehrlich Ascites Carcinoma Model
Hussein, J., El- Bana, M., Abdel Latif, Y., El-Sayed, S., Youssef, A., Elnaggar, M., Medhat, D. (2023). Processed Cheeses Fortified by Laurus Nobilis L. Extract Nanoemulsion Ameliorate Hyperhomocysteinemia in Ehrlich Ascites Carcinoma Model. EKB Journal Management System, 66(2), 199-211. doi: 10.21608/ejchem.2022.135198.5944
Jihan Hussein; Mona El- Bana; Yassmin Abdel Latif; Samah El-Sayed; Ahmed Youssef; Mehrez Elnaggar; Dalia Medhat. "Processed Cheeses Fortified by Laurus Nobilis L. Extract Nanoemulsion Ameliorate Hyperhomocysteinemia in Ehrlich Ascites Carcinoma Model". EKB Journal Management System, 66, 2, 2023, 199-211. doi: 10.21608/ejchem.2022.135198.5944
Hussein, J., El- Bana, M., Abdel Latif, Y., El-Sayed, S., Youssef, A., Elnaggar, M., Medhat, D. (2023). 'Processed Cheeses Fortified by Laurus Nobilis L. Extract Nanoemulsion Ameliorate Hyperhomocysteinemia in Ehrlich Ascites Carcinoma Model', EKB Journal Management System, 66(2), pp. 199-211. doi: 10.21608/ejchem.2022.135198.5944
Hussein, J., El- Bana, M., Abdel Latif, Y., El-Sayed, S., Youssef, A., Elnaggar, M., Medhat, D. Processed Cheeses Fortified by Laurus Nobilis L. Extract Nanoemulsion Ameliorate Hyperhomocysteinemia in Ehrlich Ascites Carcinoma Model. EKB Journal Management System, 2023; 66(2): 199-211. doi: 10.21608/ejchem.2022.135198.5944

Processed Cheeses Fortified by Laurus Nobilis L. Extract Nanoemulsion Ameliorate Hyperhomocysteinemia in Ehrlich Ascites Carcinoma Model

Egyptian Journal of Chemistry
Volume 66, Issue 2, February 2023, Page 199-211  XML PDF (1.12 MB)
Document Type: Original Article
DOI: 10.21608/ejchem.2022.135198.5944
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Authors
Jihan Hussein email orcid 1; Mona El- Bana2; Yassmin Abdel Latif3; Samah El-Sayed4; Ahmed Youssef5; Mehrez Elnaggar6; Dalia Medhat7
1Medical Biochemistry Dept.,National Research Centre,Giza,Egypt.
2Medical Biochemistry Dept., National Research Centre
3Medical Biochemistry Deart., National Rsearch Centre, Cairo,Egypt.
4Dairy Science Department, National Research Centre, Dokki, Giza
5Packing and Packaging Materials Department, National Research Centre
6Textile Research and Technology Institute, National Research Center
7Medical Biochemistry Dept.,National Research centre
Abstract
We aimed to evaluate the protective effect of processed cheeses fortified by Laurus nobilis L. extract nanoemulsion against hyperhomocysteinemia in Ehrlich ascites carcinoma (EAC) model. Laurus nobilis L. extract nanoemulsion was prepared via spontaneous emulsification procedure. Then, nanoemulsion was added by different loadings to supplement processed cheeses. The morphology of Laurus nobilis L. extract nanoemulsion was detected using transmission electron microscopy (TEM). Female Swiss albino mice received processed cheese for two weeks then inoculated once with EAC cells. After the end of the experiment, blood samples were collected for determination of serum superoxide dismutase (SOD) , thiobarbituric acid reactive substances (TBARS), B-cell lymphoma 2 (Bcl-2), matrix metallopeptidase-9 (MMP-9), Tumor necrosis factor-α (TNF-α), and homocysteine (Hcy). Results revealed that the formation of Laurus nobilis L. extract nanoemulsion in spherical shape in size around 50 -120 nm. Mice inoculated with EAC cells showed significant increase in serum Hcy, TNF-α, TBARS, and MMP-9 levels while there was a significant decrease in SOD activity and Bcl-2 level. Pretreatment with different concentrations of Laurus nobilis L. extract significantly attenuated oxidative stress, inflammation, and induced apoptosis compared to EAC group.
Keywords
Laurus nobilis L. extract nanoemulsion; Homocysteine; B-cell lymphoma 2; Ehrlich ascites carcinoma; HPLC
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