Ameliorative effect of N acetyl cysteine against diclofenac-induced oxidative stress, hematological and splenic toxicity in male rats
kasem, S., Helal, M., mahmoud, S., salama, E., Elkady, A., Elsayed, R. (2022). Ameliorative effect of N acetyl cysteine against diclofenac-induced oxidative stress, hematological and splenic toxicity in male rats. EKB Journal Management System, 10(1), 1-13. doi: 10.21608/esctj.2022.127050.1006
sara Elsayed kasem; Maha Helal; sheren mahmoud; Eman salama; Aya Elkady; Reda M Elsayed. "Ameliorative effect of N acetyl cysteine against diclofenac-induced oxidative stress, hematological and splenic toxicity in male rats". EKB Journal Management System, 10, 1, 2022, 1-13. doi: 10.21608/esctj.2022.127050.1006
kasem, S., Helal, M., mahmoud, S., salama, E., Elkady, A., Elsayed, R. (2022). 'Ameliorative effect of N acetyl cysteine against diclofenac-induced oxidative stress, hematological and splenic toxicity in male rats', EKB Journal Management System, 10(1), pp. 1-13. doi: 10.21608/esctj.2022.127050.1006
kasem, S., Helal, M., mahmoud, S., salama, E., Elkady, A., Elsayed, R. Ameliorative effect of N acetyl cysteine against diclofenac-induced oxidative stress, hematological and splenic toxicity in male rats. EKB Journal Management System, 2022; 10(1): 1-13. doi: 10.21608/esctj.2022.127050.1006

Ameliorative effect of N acetyl cysteine against diclofenac-induced oxidative stress, hematological and splenic toxicity in male rats

Egyptian Society of Clinical Toxicology Journal
Article 1, Volume 10, Issue 1, June 2022, Page 1-13  XML PDF (1.43 MB)
Document Type: Original Article
DOI: 10.21608/esctj.2022.127050.1006
View on SCiNiTO View on SCiNiTO
Authors
sara Elsayed kasem email 1; Maha Helal2; sheren mahmoud3; Eman salama4; Aya Elkady5; Reda M Elsayed6
1Forensic science and clinicl toxicology,faculty of medicine,sohag university
2Forensic Medicine and Clinical Toxicology Department, Faculty of Medicine, Sohag University. Sohag, Egypt
3Pathology,faculty of medicine,sohag university
4Clinical Pathology Department,Faculty of Medicine, Sohag University. Sohag, Egypt
5forensic science and clinicl toxicology,faculty of medicine,sohag university
6Department of forensic medicine and clinical toxicology, faculty of medicine Sohag university
Abstract
Diclofenac sodium is one of the non-steroidal anti-inflammatory drugs that is used as anti-inflammatory, analgesic, and antipyretic. N-acetyl cysteine is an antioxidant and free radical scavenger used to combat oxidative stress-induced damage in various tissues. Objectives: The current study was designed to investigate diclofenac sodium toxic effects upon the blood parameters, oxidative stress marker and spleen after repeated oral dose and evaluate the possible protective effect of N- acetyl cysteine when co-administered with it. Methods: Forty adult male albino rats were divided equally into four groups. Group I: the rats were fed on basal diet and distilled water. Group II received N-acetyl cysteine orally at the dose of 150 mg /kg /day. Group III: the rats were treated orally with diclofenac sodium at dose 5 mg /kg /day. Group IV received diclofenac sodium and N-acetyl cysteine at the same mentioned dose. After one month ,all animals were sacrificed and blood was collected for analysis. The spleen was preserved for histopathological examination. Results: The study proved that repeated oral administration of diclofenac sodium induced significant toxic effect on blood parameters, increased serum malondialdhyde level and histopathological changes in spleen. But these toxic effects declined markedly with N- acetyl cysteine co-administration. Conclusions: The present study concluded that diclofenac sodium has many toxic effects on blood parameters, induces oxidative stress and histopathological alteration in spleen tissues. Also, the study revealed that N- acetyl cysteine has a potential protective effect against such harmful effects.
Keywords
Diclofenac sodium; N- acetyl cysteine, blood parameters, MDA, spleen
Statistics
Article View: 452
PDF Download: 344