In Vitro Comparative Study on the Effect of Free Alcohol Mouth Wash Versus 10% Alcohol Based Mouth Wash on Oral Epithelial Cells Using Transformed Cell Line GMSM-K | ||||
| Egyptian Journal of Histology | ||||
| Article 21, Volume 46, Issue 3, September 2023, Page 1326-1331 PDF (746.07 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejh.2022.132835.1672 | ||||
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| Authors | ||||
Shaimaa Ali hamouda   1; Reham S Hamed2
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| 1Oral and maxillofacial pathology, Cairo University, Egypt | ||||
| 2oral and maxillofacial pathology, Cairo University, Egypt | ||||
| Abstract | ||||
| Introduction: Maintaining a good oral hygiene is very critical for the quality of life. The use of mechanical method alone to preserve the oral hygiene was insufficient activity. Therefore, various types of mouthwashes are available for use as daily oral hygiene measure. Several researches were examining the antibacterial effect of these agents. However, there are limited data about the cytotoxicity of these mouthwashes on epithelial cells. The target of the present study is to detect the toxic effect of zero alcohol mouth rinse with essential oils and sodium fluoride (EOF) compared with alcohol based mouthwash containing essential Oils (Eos) and chlorhexidine (CHX) mouthwash which used as positive control on GMSM-K cultured epithelial cells. Material and Material and Methods: Zero alcohol mouth rinse with EOF, alcohol-based mouth rinse combined with EOs and CHX were applied to the epithelial cell line at 10%, 35% and 75% concentrations. The toxic effect of the mouthwashes was assessed by MTT assay to detect cell viability, flow cytometry using annexion V gene in order to detect cell apoptosis and necrosis and finally, by measuring the production of interleukin (IL)-6 by enzyme-linked immunosorbent assay. Results: By analyzing the obtained data using ANOVA statistical test, it was found that the mouthwash with alcohol and Eos had the strongest toxic effect on epithelial cells in concentration dependent manner with statistically significant difference at 75% concentration followed by CHX and zero alcohol EOF mouthwashes respectively. Conclusion: It was found that zero alcohol mouth rinse including EOF was the safest mouthwash on the epithelial cells. | ||||
| Keywords | ||||
| Chlorhexidine; epithelial cell; essential oils; mouthwash containing alcohol; sodium fluoride | ||||
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