The Effect of Recombinant Human Bone Morphogenetic Protein-7 (rhBMP-7) on the osteoblast-like cells cultured on implant titanium discs
N., M., A., A., A., D., A., A. (2021). The Effect of Recombinant Human Bone Morphogenetic Protein-7 (rhBMP-7) on the osteoblast-like cells cultured on implant titanium discs. EKB Journal Management System, 32(3), 44-46. doi: 10.21608/mjmr.2021.241643
Mahmoud N.; Ahmed A.; Dina A.; Abdullah A.. "The Effect of Recombinant Human Bone Morphogenetic Protein-7 (rhBMP-7) on the osteoblast-like cells cultured on implant titanium discs". EKB Journal Management System, 32, 3, 2021, 44-46. doi: 10.21608/mjmr.2021.241643
N., M., A., A., A., D., A., A. (2021). 'The Effect of Recombinant Human Bone Morphogenetic Protein-7 (rhBMP-7) on the osteoblast-like cells cultured on implant titanium discs', EKB Journal Management System, 32(3), pp. 44-46. doi: 10.21608/mjmr.2021.241643
N., M., A., A., A., D., A., A. The Effect of Recombinant Human Bone Morphogenetic Protein-7 (rhBMP-7) on the osteoblast-like cells cultured on implant titanium discs. EKB Journal Management System, 2021; 32(3): 44-46. doi: 10.21608/mjmr.2021.241643

The Effect of Recombinant Human Bone Morphogenetic Protein-7 (rhBMP-7) on the osteoblast-like cells cultured on implant titanium discs

Minia Journal of Medical Research
Volume 32, Issue 3, July 2021, Page 44-46  XML PDF (218.75 K)
Document Type: Original Article
DOI: 10.21608/mjmr.2021.241643
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Authors
Mahmoud N.1; Ahmed A.2; Dina A.3; Abdullah A.4
1Oral Medicine, Oral Diagnosis and Periodontology, Cairo University- Egypt.
2Oral Medicine, Oral Diagnosis and Periodontology, Faculty of Dentistry, Minia University.
3Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University
4Oral Medicine, Oral Diagnosis and Periodontology, Faculty of Dentistry, Minia University
Abstract
Aim: Was to study the effect of recombinant human bone morphogenetic protein-7 (rhBMP7) on the osteoblast-like cells cultured on implant titanium discs. Methodology: The 
osteoblast-like Osteo-1 cell line was used in this experiment and derived from parietal bone 
tissue of newborn albino rats. The cells was incubated in a humid atmosphere of 95% air and 
5% carbon dioxide at 37°C. The medium was changed every 2 days. Four groups were 
conducted as follow Osteoclast like cell (Machined), Implanted Osteoclast like cell on 
Titanium (Modified), Osteoclast like cell supplemented with BMP-7 (Machined+ BMP-7) 
and Implanted Osteoclast like cell on Titanium and supplemented with BMP-7 
(Modified+BMP-7). Results: Cell proliferation was influenced by rhBMP-7, as demonstrated 
by a significant increase in collagen content after 7 and 21 days of culture (P =0.005) and a 
significant increase in alkaline phosphatase activity after 7 days (P <0.001). The addition of 
rhBMP-7 influenced alkaline phosphatase activity, and a significant increase was observed 
after 21 days (P <0.001). Conclusion: Within the limitations of the study, we concluded that 
the presence of rhBMP-7 did not influence cell adhesion to chemically modified titanium 
surfaces but provided an additional stimulus during the differentiation of rat osteo-1 cells 
cultured on this type of surface.

Keywords
Recombinant BMP; Osteoclast like cells; implant
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