INVESTIGATION OF MORPHOLOGICAL AND ANATOMICAL CHANGES IN CATHARANTHUS ROSEUS (L.) G. DON DUE TO COLCHICINE INDUCED POLYPLOIDY | ||||
| Scientific Journal of Flowers and Ornamental Plants | ||||
| Article 2, Volume 5, Issue 3, September 2018, Page 233-243 PDF (506.14 K) | ||||
| Document Type: Original Research Article | ||||
| DOI: 10.21608/sjfop.2018.24216 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Awad Shala1; Z. Deng2 | ||||
| 1Medicinal and Aromatic Plants Research Department, Sakha Horticulture Research Station, Horticulture Research Institute. Agricultural Research center. | ||||
| 2Department of Environmental Horticulture, Gulf Coast Research and Education Center, University of Florida, USA. | ||||
| Abstract | ||||
| Catharanthus roseus (L.) G. Don is valuable medicinal plant grown as an ornamental plant. The study was carried out at Gulf Coast Research and Education Center, University of Florida, USA. to evaluate the morphological and anatomical changes from induced polyploidy in periwinkle for this purpose colchicine was applied at four different concentrations 0.0, 0.05, 0.1 and 0.2% for seedling treatment. The results showed that raising colchicine doses caused an increasing of survival percentage and tetraploid plants number. Polyploids showed an increment in leaf thickness, stomatal size, pollen diameter and pollen viability compared to diploid plants. Flow cytometry verified the existence of tetraploids and mixoploids from various colchicine-treated plants especially 0.2 which was found efficient on inducing polyploidy in the plants followed by 0.1% as compared to other concentration under study. | ||||
| Keywords | ||||
| Vinca; chromosome doubling; Colchicine; nuclear DNA content; tetraploid | ||||
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