EFFECT 0 F VITAMIN E (ALPHA-TOCOPHEROL) ADDITION TO RABBIT SEMEN on SURVIVAL AND FERTILITY | ||||
| Journal of Animal and Poultry Production | ||||
| Article 2, Volume 28, Issue 1, January 2003, Page 105-114 PDF (2.72 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jappmu.2003.242174 | ||||
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| Authors | ||||
| W. Kishk1; S. Yousif2; J. Rafay3 | ||||
| 1Suez Canal University, Faculty of Agriculture, Animal Production Dept., Ismailia, Egypt | ||||
| 2Veterinary Research Institute, Ministry of Agriculture, Ismailia, Egypt | ||||
| 3Research Institute of Animal Production, 949 92 Nitra, Hlohovska 2, Slovakia | ||||
| Abstract | ||||
| Five Nitra male rabbits were subjected to artificially semen collection. Samples with percent motility above 70 were pooled for subsequent dilution and processing. Pooled semen samples were diluted (1 :5) with Tris extender free of egg- yolk. Three different levels of vitamin E were added to extended semen samples. These levels were 2, 4, 8 mg vitamin E for each 5 ml extended semen in addition to two control groups (control 1 without vitamin E and control 2 with ethanol and without vitamin E), respectively. Diluted samples with different levels of vitamin E were kept at 15°C for one day. Sperm motility, live and dead sperm, broken head and broken tail sperm percentages, and acrosome integrity criteria were measured for diluted samples of different groups after dilution by 0, 2 and after 24 hours of incubation at the same temperature. It was found that vitamin E addition significantly improved sperm motility % after two hours 0 f incubation a t a II a dded I evels. In which sperm motility were 70% for different levels of vitamin E and 65% for control 1 & 2 groups, respectively after 2 h rs incubation periods. A Iso acrosome integrity % was differed among different groups. Where, it was higher in correlation with higher doses of vitamin E. Average values of acrosome integrity % (A.I) were 91.67, 92.00 and 95.33 for three different levels of vit E respectively. Whereas, they were (A.I) 81.33 and 79.33 for control 1 and control 2, respectively. As for live and dead sperm percentage, broken head and broken tail sperm, did not differ significantly according to different groups. It could be concluded that, vitamin E can be included in rabbit semen diluters as a sperm metabolism protectant especially for fine organelles and structures such as galea caputis (acrosome). In addition, fertility rate for different levels of vitamin E addition to extended semen was 75, 75, 70 and 85 % for control, first, second and third level, respectively. | ||||
| Keywords | ||||
| Semen; rabbit sperm; vitamin E; acrosome reaction; motility; fertility | ||||
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