EFFECT OF USING TWO METHODS OF STAINING ON SPERM MORPHOLOGY CHARACTERS OF BULL AND BUCK SPERM AFTER FREEZING. | ||||
Journal of Animal and Poultry Production | ||||
Article 3, Volume 28, Issue 1, January 2003, Page 115-128 PDF (3.71 MB) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/jappmu.2003.242175 | ||||
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Author | ||||
W. H. Kishk, | ||||
Animal Production Department, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt. | ||||
Abstract | ||||
Two different methods of staining (Harris hematoxylin&Modified Bryan's sperm stain) were used to explore sperm morphology changes after freezing for both bull and buck sperm. Morphology sperm characters were concerned with state of the following sperm organelles; head, med piece, acrosome, tail and cytoplasmic droplet. Observations of abnormal sperm morphology were higher and differed significantly for rabbit sperm than bull sperm after freezing. In addition both bull, buck sperm morphology differed significantly before and after freezing. It couid be concluded that, rabbit sperm is more sensitive for freezing process than bull sperm and it is still needed for more appropriate freezing media to avoid freezing injuries at cellular and sub cellular levels. | ||||
Keywords | ||||
Rabbit; Buck; Bull; Sperm; Staining; Acrosome; Frozen semen | ||||
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