MICROBIAL PRODUCTION OF INVERTASE AND ITS --- UTILIZATION IN· PREPARATION OF INVERTED SUGARS | ||||
| Journal of Food and Dairy Sciences | ||||
| Article 1, Volume 28, Issue 1, January 2003, Page 401-411 PDF (3.06 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jfds.2003.242207 | ||||
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| Authors | ||||
| Azza A. Bakry,1; M. A. Salem2; Badeaa A. Bessar2 | ||||
| 1Dept. of Special !=dO'd' a Nutr~ ~ood Tech, Res. Inst., ARC, Giza, Egypt | ||||
| 2Food Tech d~pt., FaelilftlY 6f AgtM" Kafr EI-Sheikh, Tanta Univ. | ||||
| Abstract | ||||
| The present investigation was carried out to clarify the feasibility of invertase production from some agricultural WcNtes (chicory roots, lettuce leaves, grass, cabbage top, potato leaves and sweet potato leaves.) using Asp < /em>ergillus oryza NRRL 451 and Saccharomyas cerevisiae CBS 1200. The optimal conditions for enzyme production and action were determined. The hydrotytic efficiency of enzyme for hydrolysis sucrose to produce inverted sugar was studied. Also the physicochemical properties of syrups produced were studied. Results indicated that invertase is successfully produced by Aspergillus oryzae NRRL$S1 and Saccharomyces cerevisiae CBS 1200 using· all tested substrates as carbon sources. The maximum production of invertase (5.31 and 2.79 units/ml medium) were recovered by cultivating Aspergillus oryzae NRRL$S1 and Saccharomyces cerevisiae CBS 1200 on a media contains 2.5 and 3% grass powder at pH 5.0 and 5.S after 72 and 48 hours of fer.mentation at 30°C respectively. The maximal activity of enzyme was at pH rangeS.Oto 5.5and45t050oC,whilethe enzyme was stable at pH values from 4.5 to 7.0 and below 50°C. It was inhibited by Ag and H g. Invertase produced by A spergil/us 0 ryzae N RRL$S1 has high hydrolytic efficiency (95.22%) and it could be recommended for production of inverted sugar on commercial scale. | ||||
| Keywords | ||||
| agricultural wastes; invertase; Aspergillus oryza; Saccharomyces cerevisiae; inverted sugar | ||||
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