PLANT REGENERATION FROM LEAF BLADE, PEDICLE AND SHOOT TIP OF CULANTRO (Eryngium foetidum L.), A MEDICINAL PLANT | ||||
Journal of Plant Production | ||||
Article 11, Volume 28, Issue 2, February 2003, Page 885-892 PDF (5.96 MB) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/jpp.2003.242254 | ||||
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Author | ||||
Yasseen, Y. Mohamed | ||||
Genetic Engineering and Biotechnology Research Institute Sadat City, P.O, Box 79122857, Minufiya University, Egypt | ||||
Abstract | ||||
Culantro is an aromatic plant. which is used as condiment and medicinal plant. A procedure for shoe; regeneration (rom leaf blade, pedicle and shoot tip explants, is described. Leaf blade, pedicle and shool tip explants were cultured on Murashige and Skoog medium (MS) alone or supplernentert with 0,5,1,2,3,4,5,6 mgll benzyladenine (SA) with 0.5 mgll naphlhaleneacetic acid (NAA). Shoot regeneration from the proximal third section of leaf blade was higher than shoot regeneration from the middle and distal sections. Shoot regeneration, shoot length and roots number were innuenced by SA and NAA concentrations. Shoot regeneration from quartered shoot tip was higher than shoot regeneration from decapitated or intact shoot tip. Produced shoots were transferred into root- induction medium and rooted shoots were successfully established in soil. Abbreviations: SA, benzyladenine; MS, Murashige & Skoog's (1962) medium; NAA • naphthaleneacetic acid. | ||||
Keywords | ||||
Tissue culture; herb; medicinal plant; coriander substitute; herbs and spices | ||||
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