EFFECT OF GAMMA IRRADIATED SEEDS OF Phaseo/us vulgaris ON THE GENOSYMBIOTIC EFFICIENCY OF Rhizobium leguminosarum BIOVAR PHASEOLI AFFECTING VEGETATIVE AND SOME CHEMICAL TRAITS IN Phaseo/us vulgairs | ||||
| Journal of Plant Production | ||||
| Article 13, Volume 28, Issue 2, February 2003, Page 907-930 PDF (19 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2003.242273 | ||||
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| Authors | ||||
| K. A. Zaied,; Z. A. Kosba; A. H. Abd EI-Hady; S. S. M. EI-Sayed | ||||
| Dept. of GenetIcs, Fac. of Agric., Mansoura Unlv. | ||||
| Abstract | ||||
| Seeds from two varielies of common bean (Phaseo/us vulgaris) were irradiated with four doses of gamma rays to induce mutations affecting Rhizobium symbiosis. as the development of N2"fixing nodules requires a coordinated expression of genes of both symbiotic partners. Symbiotic response to irradiation affecting leguminous host was measured in two generations using wild type strain oi rhizobia. Both doses of 20 and 40 Krad revealed higher symbiotic efficiency in M. and M~ populations. respectively. Inoculation with rhizobia was shown to decrease the deleterious effect of radiation below that of un inoculated ones. Gamma irradiation disrupted the normal ontogeny leading to reduction the development of new tissues in some vegetative traits. leaf area was markedly reduced by gamma irradiation, and was suitable for radiosensitivity assay depending on the growth of genotypes. Leaf area was significantly affected by both doses of gamma irradiation and biofertilization among M, and ~ populations. Protein content and chlorophyll concentrations in most of \he inoculated plants was higher than uninoculated ones. Plants inoculated with rhizobia produced higher seed protein content than uninoculated ones. The interaC\!on between varieties by biofertilization significantly affected shoot nitrogen content among M, and M2 populations. Pod productivity in both generations was markedly affected by ptant genotypes, doses of gamma rays and biofertilization. Reductions in seed and pod yields I plant were observed in M, and M2 populations as dosages of gamma rays progressed. The results indicated that plant is most sensitive to gamma irradiation from the time it begins to develop its reproductive capability until after fertilizaUon when embryo development begins. Irradiation was used in this study to induce genetic variatioo in common bean to enhance the response of rhizobia and the legume host to increase N, fixation. | ||||
| Keywords | ||||
| Chlorophyll. gamma rays; Phaseo/us vulgaris; RhiZobium; syrnolotic efficiency | ||||
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