EFFECT OF PUTRESCINE AND PHENYLALANINE ON GROWTH, AND ALKALOID PRODUCTION OF SOME Datura species. | ||||
| Journal of Soil Sciences and Agricultural Engineering | ||||
| Article 5, Volume 29, Issue 7, July 2004, Page 4307-4323 PDF (5.2 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jssae.2004.242505 | ||||
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| Authors | ||||
| A. A. Youssef1; R. A. EI-Mergawi2; M. S. Abd EI Wahed2 | ||||
| 1Cultivation and Production Medicinal and Aromatic Plants, Dept. NRC, Dokki, Cairo | ||||
| 2Botany Dept., NRC; Dokki, Egypt | ||||
| Abstract | ||||
| Two field experiments were carried out at the Experimental Farm of Cultivation and Production of Medicinal and Aromatic Plants Dept. National Research Centre at Giza, during two successive seasons, to study the effect of foliar application of two tropane alkaloid precursors, phenylalanine (0, 100 and 200 ppm) and/or putrescine (0, 50 and 100 ppm) on some growth parameters and alkaloid contents of D. stramonium and D. innoxia plants that originated from Bulgaria. Datura stramonium showed the superiority of all growth parameters, except root weight at flowering stage, while at fruiting stage. Datura innoxia showed visible increment of all growth parameters. Application of phenylalanine 100 ppm+ putrescine 50 ppm was sufficient to accelerate the growth parameters of D. stramonium at flowering and fruiting stages and D innoxia at fruiting stage. At flowering stage, significant promotion in all growth parameters of D. innoxia was observed due to foliar application of phenylalanine 100+ putrescine 100 ppm. D. stramonium with high level of phenylalanine and putrescine separately significantly increased crude protein in leaves at flowering stage, while D. innoxia had an opposite trend. The highest value of free amino acids in leaves at flowering stage was obtained from D. stramonium plants treated with putrescine 50 ppm, while the maximum percentage for D. innoxia was observed in plants treated with phenylalanine 100+ putrescine100 ppm. Hyoscine and hyoscyamine alkaloids were determined in leaves and fruits at flowering and fruiting stages using HPLC. Most of phenylalanine and putrescine treatments tended to increase hyoscine and hyoscyamine contents (mg/g dry matter and mg/plant) of two Datura spp, throughout the growing seasons. Foliar spraying with phenylalanine and putrescine connectively increased greatly hyoscine, hyoscyamine and total alkaloids contents in Datura spp more than individually ones, in most cases. Application of phenylalanine 100 + putrescine 50 ppm gave the highest values of hyoscyamine mg/plant, while treatment of phenylalanine 100 + putrescine100 gave the highest levels of total alkaloids mg Iplant in both Datura spp. | ||||
| Keywords | ||||
| Datura stramonium; Datura innoxia; Polyamines; Putrescine; Phenylalanine; Growth; Yield; Alkaloids; Hyoscine; Hyoscyamine | ||||
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