EFFECT OF GAMMA RAYS, ABSCISIC ACID AND PUTRESCINE ON PRODUCTION OF WHEAT PLAN I TS MORE TOLERANT TO SALINITY: ~ B· IN VITRO CALLUS INDUCTION, PLANT REGENERAliION, AND GRAINS PRODUCTION' UNDER SALINE CONDITIONS | ||||
| Journal of Plant Production | ||||
| Article 10, Volume 28, Issue 5, May 2003, Page 3551-3570 PDF (3.59 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2003.243296 | ||||
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| Authors | ||||
| Y. H. EI-Shafey,; S. M. Salem; O. M. EI-Shihy; A. M. Ghallab; Hanaa F. Y. Mohamed | ||||
| Plant Physlo!. Sec., Fac. of Agric., Cairo unlv., Egypt. | ||||
| Abstract | ||||
| In vitro tlssue culture experiment was conducted to e valuate the ext nt of improvement in salinity tolerance of the sensitive Giza 167 cultivar due l~1 the previously applied treatments in t he pot experiments during t he first part (A) ~ f the present investigation. The embryonic calli, plantlets and plants regenerated fro I the grains produced either by the salt-sensitive untreated and treated plants of Giz 167 cullivar or by the untreated plants of Sakha 8 one. in the second pot expejl'ment season; 2000·2001 were used to be tested in vitro under the same salinity eve Is previously applied in the first part of this study. The obtained results from the in vitro experiments were in full accordance with those obtained in the first part, as repards the absolute superiority of the regenerated calli, plantlets, and plants from the "lature grains of the previously treated plants (in the pot experiments) with the weekly spraying either with 10IJM putrescine or 2 ppm ABA as well as the grains irradiatit n with 15 KR gamma rays in their growth, chemical composition (In both tissues and grains), and invertase activity as well as their grain yield (g)fplant and 1000 gtains. weight which recorded the highest highly significant increments over the respective values of those produced from the grains of the satt-tolerant Sakha 8 control, al~?, up to 30% sea water level, nevertheless, without applying any treatments under such In vitro conditions. This finding strongly Indicating the possibility of transmission at the phYSiological tolerance to salinity induced by those treatments previously applied in the pot experiments in the first part with nearly equal extent from the parent tolerant plants to their regenerations under in vitro conditions, thus enable them to be ad~pted to the all applied levels of salinity in the media and therefore, could complete th:t life cycle till harvest and grains production which were highly significantly exceeded that both controls a Iso up to 30% sea water under in vitro conditions, as d ld u nder pet experiments conditions in the first part. Therefore, the obtained results strongly suggested that the tolerance to salinity of the salt-sensltive Giza 167 wheat cu tivar potted plants and even their regenerated plants under in vitro conditions, can be improved to a considerable extent, thus could tolerate the irrigation with saline ater up to 30% sea water and nevertheless, attained their optimal productivity which h ghly significantly exceed the productivity of the salt-tolerant Sakha 8 cultivar control under the same 30% salinity level in both cases, if these sensitive plants were weekly sprayed either with 10 IJM putrescine or 2 ppm ABA or when their grains were irradiated with 1 5 K R gamma rays before s owing only in the pot experiments, ith special referring to the putrescine treatment in this concern. | ||||
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