In-house production of Fluorescence in situ hybridization probes for alpha-satellite centromeric region for detection of chromosomal aneuploidy
Hussein, S., Amr, K., Abd El-Fattah, A., Mohamed, A. (2022). In-house production of Fluorescence in situ hybridization probes for alpha-satellite centromeric region for detection of chromosomal aneuploidy. EKB Journal Management System, 2(2), 131-138. doi: 10.21608/aijpms.2022.121845.1114
Shymaa H Hussein; Khalda S Amr; Abeer I Abd El-Fattah; Amal M Mohamed. "In-house production of Fluorescence in situ hybridization probes for alpha-satellite centromeric region for detection of chromosomal aneuploidy". EKB Journal Management System, 2, 2, 2022, 131-138. doi: 10.21608/aijpms.2022.121845.1114
Hussein, S., Amr, K., Abd El-Fattah, A., Mohamed, A. (2022). 'In-house production of Fluorescence in situ hybridization probes for alpha-satellite centromeric region for detection of chromosomal aneuploidy', EKB Journal Management System, 2(2), pp. 131-138. doi: 10.21608/aijpms.2022.121845.1114
Hussein, S., Amr, K., Abd El-Fattah, A., Mohamed, A. In-house production of Fluorescence in situ hybridization probes for alpha-satellite centromeric region for detection of chromosomal aneuploidy. EKB Journal Management System, 2022; 2(2): 131-138. doi: 10.21608/aijpms.2022.121845.1114

In-house production of Fluorescence in situ hybridization probes for alpha-satellite centromeric region for detection of chromosomal aneuploidy

Azhar International Journal of Pharmaceutical and Medical Sciences
Article 13, Volume 2, Issue 2, June 2022, Page 131-138  XML PDF (292.02 K)
Document Type: Original research articles
DOI: 10.21608/aijpms.2022.121845.1114
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Authors
Shymaa H Hussein email 1; Khalda S Amrorcid 2; Abeer I Abd El-Fattah3; Amal M Mohamed1
1Department of Human Cytogenetics, Institute of Human Genetics and Genome Research, National Research Centre, Cairo, Egypt
2Deparment of Medical Molecular Genetics, Institute of Human Genetics and Genome Research, National Research Centre, Cairo, Egypt
3Department of Biochemistry, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo, Egypt
Abstract
Fluorescence in situ hybridization (FISH) depends on the complementary nature of the labeled probe and its targeted chromosomal segment. FISH technique allows the detection of numerical chromosomal aneuploidy by using the centromeric probes. Numerical aberrations represent a significant percentage of chromosomal abnormalties and is a main cause of pregnancy loses and cancer. The present study aimed to produce in-house FISH centromeric probes that help in the identification of numerical chromosomal aberrations (copy number variants) and can be used as a control probe. In this study, the probes were produced using Polymerase Chain Reaction (PCR) with specific primers and human genomic DNA. PCR was used for amplification and labeling of alpha satellite centromeric probes of chromosomes 1 and 7. The alpha satellite centromeric probes allow us to identify the numerical abnormalities of its corresponding chromosome. All the produced probes were sensitive and specific for the detection of chromosomal copy number variants. To our knowledge this is the first time to produce FISH probes in Egypt, our next plan is to produce double colour probes for specific locus.
Keywords
Fluorescence In Situ Hybridization (FISH); alpha satellite centromeric probe; aneuploidy; copy number variants (CNV); probe production
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