DEGUMMING OF SILK FIBERS BY PROTEASE FR' M Bacillus subtilis | ||||
| Journal of Agricultural Chemistry and Biotechnology | ||||
| Article 3, Volume 28, Issue 6, June 2003, Page 5031-5041 PDF (1.18 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jacb.2003.244695 | ||||
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| Authors | ||||
| N. A. Neweigy,; H. E. Abou Aly | ||||
| Agric. Botany Dept. Fac. Agrlc. Moshtoho" Zagazlg Unlv., Egypt | ||||
| Abstract | ||||
| Four stralns of Bacillus subtifis (MZ. M14, M35 and M54) were selected. for production ot protease enzyme and degumming of silk fibers. The data revealed that. maximum protease activity and specific activity were obtained by B. subtifis(M14) followed by' B. subtilis(M54) when grown in Chopra and Malhur medium al 30°C for 5 days under shake culture conditions. When the strains individually incubated In Ihe medium supported with silk fiber. the loss of the gum might amnl!nt to 30.56%' i!h high lotal activity of protease In Ihe culture supematants. Culture supernatants of four strains with high protease activity were most effective in reducing the gum content of silk fibe~ within 4 Iv and slightly Increase within 8 and 16 hr. Four grams silk fiber 'Per 100 ml culture filtrate was the best ratio for the highest degumming by B. subtilis M14, M35 and M54 strains while 3:100 (wN) was the best ratio tor degumming by B. subtills (M2). Degumming or silk fibers increased with the using of erude e!l2.yme of B. subtilis (M14) followed by (M54) and (M2) strains at 30 ·C, while B, subtilis (M35) gave ~the highest silk degumming at saoc. Results showed that, maximum degumming and total aQMty of protease have been observed at pH 7.0 to all filtrates of the four strains. These results indicated that, protease of B. suM/is plays an active role in he degumming or silk libe~. | ||||
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