Bioengineering of Zinc Oxide Nanoparticles As Therapeutics for Immunomodulatory and Antimicrobial Activities
El-Sherebiny, G., Ali, A., Ali, D., El-Hawary, A., Asker, A. (2022). Bioengineering of Zinc Oxide Nanoparticles As Therapeutics for Immunomodulatory and Antimicrobial Activities. EKB Journal Management System, 65(131), 1473-1486. doi: 10.21608/ejchem.2022.136366.6008
Gamal Mohamed El-Sherebiny; Amir R. Ali R. Ali; Diaa Mohamed Ali; Ahamed S. El-Hawary; Ahamed A Asker. "Bioengineering of Zinc Oxide Nanoparticles As Therapeutics for Immunomodulatory and Antimicrobial Activities". EKB Journal Management System, 65, 131, 2022, 1473-1486. doi: 10.21608/ejchem.2022.136366.6008
El-Sherebiny, G., Ali, A., Ali, D., El-Hawary, A., Asker, A. (2022). 'Bioengineering of Zinc Oxide Nanoparticles As Therapeutics for Immunomodulatory and Antimicrobial Activities', EKB Journal Management System, 65(131), pp. 1473-1486. doi: 10.21608/ejchem.2022.136366.6008
El-Sherebiny, G., Ali, A., Ali, D., El-Hawary, A., Asker, A. Bioengineering of Zinc Oxide Nanoparticles As Therapeutics for Immunomodulatory and Antimicrobial Activities. EKB Journal Management System, 2022; 65(131): 1473-1486. doi: 10.21608/ejchem.2022.136366.6008

Bioengineering of Zinc Oxide Nanoparticles As Therapeutics for Immunomodulatory and Antimicrobial Activities

Egyptian Journal of Chemistry
Volume 65, Issue 131, December 2022, Page 1473-1486  XML PDF (1.07 MB)
Document Type: Original Article
DOI: 10.21608/ejchem.2022.136366.6008
View on SCiNiTO View on SCiNiTO
Authors
Gamal Mohamed El-Sherebiny email orcid 1; Amir R. Ali R. Ali2; Diaa Mohamed Ali3; Ahamed S. El-Hawary4; Ahamed A Asker4
1Prof. of medical microbiology,Botany and Microbiology Department, Faculty Science Al-Azhar University
22Applied-Science & Robotics Laboratory for Applied-Mechatronics (ARAtronics Lab.); Mechatronics Engineering Department, German University in Cairo, New Cairo 11835, Egypt.
3Department of Botany and Microbiology, Faculty of Science, Al-Azhar University,11884 Nasr City, Cairo, Egypt
4Ph.D. Affiliation: Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo 11884, Egypt.
Abstract
In the present work, eco-friendly biosynthesis of zinc oxide nanoparticles (ZnONPs) and their immunostimulatory and antimicrobial activity were investigated. Streptomyces rochei, the ZnONPs manufacturer, were isolated and identified. The morphology and characterization of ZnONPs using UV-Vis, DLS, TEM, and XRD, were investigated. The results showed that the spherical shape of ZnONPs was with a mean diameter of about 36nm. The viability of the HFB-4 and Vero cells was decreased to 42 and 46% respectively after treatment with a dose of 16µl/ml of ZnONPs. ZnONPs showed a significant immunostimulatory effect with 137.2 ± 0.18% and increased CD4+ and CD8+ lymphocytes level with 85.2 ± 0.07% and 25.3 ± 0.03%, respectively. Also, they exhibited an antimicrobial activity against both Gram-negative, positive bacteria and fungi with inhibition zone ranging from 16.0 to 28.0 mm and MIC ranging from 8.67 to 125.0 µg/ml. This study highlights that ZnONPs strongly stimulate immune system and have antimicrobial activities.
Keywords
Streptomyces rochei; Biosynthesis ZnONPs; Cytotoxicity; Immunomodulatory; Antimicrobial activity
Statistics
Article View: 428
PDF Download: 95