USE OF PROTEIN ELECTROPHORESIS TO QUANTIFY RESISTANCE OF FLAX CUL TIVARS TO RUST DISEASE | ||||
| Journal of Plant Production | ||||
| Article 5, Volume 28, Issue 10, October 2003, Page 7255-7262 PDF (2.24 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2003.245244 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| A. A. Aly,; E. M. Hussein; S. M. E. Zayed | ||||
| Plant Pathology Research Institute, Agricultural, Res. Center, Giza, Egypt. | ||||
| Abstract | ||||
| Six flax cultivars were evaluated for rust resistance under field conditions in 2001/2002 and 2002/2003 growing seasons. The tested cultivars could be divided into two distinct groups. The first group included the susceptible cultivars Dakota, Wilden, Williston Brown, and Cortland, while the second group included the resistant cultivars Linore and C.I. 2008. There was a significant difference (p~ 0.05) between any cultivar belonged to the first group and any cultivar belonged to the second group. However, the differences were nonsignifigant within each group. Proteins of the cultivars were separated by SDS-PAGE, and the obtained banding patterns were visualized by using the Coomassie-blue staining system. Data for rust ratings and amounts of protein fractions were entered into a computerized stepwise multiple regression analysis. Using the predictors supplied by stepwise regression, a four- factor model was constructed to predict rust severity. This model showed the rust severity differences were due largely to the protein fractions nos. 1 , 1 8, 1 5 and 8, which accounted for 99.71% of the variation in severity ratings. These results indicate that SDS-PAGE of proteins may provide a supplementary assay to greenhouse and field tests to distinguish quantitatively between rust resistant or susceptible genot/pes. | ||||
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