DNA Methylation as a Coming Clue for Age Prediction.
Morad, M., Abdelkader, A., Abdullah, O., El-Shishtawy, M., Fouda, A. (2023). DNA Methylation as a Coming Clue for Age Prediction.. EKB Journal Management System, 1(1), 13-23. doi: 10.21608/bfstj.2022.82996.1003
Marwa Mohamed Morad; Afaf Abdelmohsen Abdelkader; Omnia Alsaied Abdullah; Mohamed Ahmed El-Shishtawy; Atef Abdelaziz Fouda. "DNA Methylation as a Coming Clue for Age Prediction.". EKB Journal Management System, 1, 1, 2023, 13-23. doi: 10.21608/bfstj.2022.82996.1003
Morad, M., Abdelkader, A., Abdullah, O., El-Shishtawy, M., Fouda, A. (2023). 'DNA Methylation as a Coming Clue for Age Prediction.', EKB Journal Management System, 1(1), pp. 13-23. doi: 10.21608/bfstj.2022.82996.1003
Morad, M., Abdelkader, A., Abdullah, O., El-Shishtawy, M., Fouda, A. DNA Methylation as a Coming Clue for Age Prediction.. EKB Journal Management System, 2023; 1(1): 13-23. doi: 10.21608/bfstj.2022.82996.1003

DNA Methylation as a Coming Clue for Age Prediction.

Biannual Forensic Sciences and Toxicology Journal
Article 4, Volume 1, Issue 1 - Serial Number 202311, 2023, Page 13-23  XML PDF (622.2 K)
Document Type: Original Article
DOI: 10.21608/bfstj.2022.82996.1003
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Authors
Marwa Mohamed Morad email 1; Afaf Abdelmohsen Abdelkader2; Omnia Alsaied Abdullah3; Mohamed Ahmed El-Shishtawy2; Atef Abdelaziz Fouda4
1Benha University
2Forensic Medicine and clinical Toxicology department, Faculty of Medicine, Benha university, Egypt.
3Medical Biochemistry and Molecular Biology- Faculty of Medicine- Benha University- Egypt.
4Forensic Medicine and Clinical Toxicology Department, Faculty of medicine, Benha university, Egypt
Abstract
Abstract
Background: DNA methylation (DNAm) is a biochemical modification which occurs over the lifespan of an individual and it is a substantial constituent in the aging process. The degree of methylation was significantly related to age. So far, the ELOVL2 locus has been the most thoroughly studied age marker. This locus has been demonstrated to be reliable in ancient and fresh human bloodstains, which constitute a major source of DNA in forensic laboratories. Aim of work: The current study aimed to assess the use of DNA methylation on the ELOVL2 gene from blood samples as biomarkers for chronological age estimation using pyrosequencing in Egypt. Material and methods: 80 whole blood samples from individuals aged 18-96 years divided into 4 groups were analysed using a DNA methylation quantification assay based on bisulphite conversion and DNA pyrosequencing of 7 CpG sites in the ELOVL2. Our results display significantly strong correlation between DNAm and chronological age; the model supporting DNAm as a strong age predictor. The age prediction accuracy was most accurate in age group III (40-49 y) and was least accurate in age group IV of the elderly individuals (50-69 y) on choosing1, 2.5-, and 5-years as difference threshold.
Keywords
DNA methylation; aging; whole blood; ELVOL2
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