In-vitro biodegradation of Glyphosate using genetically improved bacterial isolates from highly polluted wastewater | ||||
| Egyptian Journal of Chemistry | ||||
| Volume 65, Issue 132, December 2022, Page 669-681 PDF (1.01 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejchem.2022.141571.6194 | ||||
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| Authors | ||||
Nivien A. Abosereh 1; Rasha G Salim  1; Ahmed F. El-Sayed 1; Maher A. Hammad2; Ghada M. Elsayed1
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| 1Department of Microbial Genetics, National Research Centre, Dokki, Giza, Egypt. | ||||
| 2Department of Plant Protection, Faculty of Agriculture, Ain Shams University, Cairo, Egypt. | ||||
| Abstract | ||||
| Due to the problems assigned by unregulated and indiscriminate applications of pesticides, adverse effects to human health, different life forms, and ecosystems were evolved. Development of technologies that guarantee their elimination in a safe, efficient, and economical way is important, among these strategies, bioremediation that overcomes the limitations of traditional methods for the disposal of hazardous compounds. An organophosphorus pesticide, Glyphosate (N-phosphono methyl glycine), was the most persistent pesticide in the Al-Jabal Al-Asfar drain water canal. Four bacterial isolates recorded a notable degradation behavior toward glyphosate with various capabilities. The PCR amplification of the 16s rDNA gene was employed to identify these bacterial isolates. They were identified as Bacillus cereus NRC1-PP, Pseudomonas alcaligenes NRC2-Gly, Pseudomonas stutzeri NRC3-8PS, and Bacillus licheniformis NRC4-1BL and deposited at GenBank. Bacillus cereus NRC1-PP, as the highest degrader, biodegraded 28.96% of glyphosate when injected in minimal salt media after ten days. Enhancement of Glyphosate biodegradation potential for Bacillus cereus NRC1-PP through physical mutagen UV radiation and chemical mutagen Ethyl methane sulfonate (EMS) was implemented. The biodegradation fitness was increased to 2.5-fold in UV-10 bacterial mutant. Protein profiles of Bacillus cereus NRC1-PP and its mutants were investigated by SDS-PAGE. Dendrogram of SDS-PAGE based on unweighted pair group method with arithmetic averages algorithm (UPGMA) divided and categorized into 2 main clusters according to similarity coefficient. The enzymatically- generated degradation products of Glyphosate by GC/MS were detected. The treated samples were presented 12 metabolites were detected in the case of UV-10 treatment; however, only presented 7 metabolites were assigned in the untreated sample (control). These metabolites included amino methyl phosphonic, and new ions such as C2N2O and C3H4O3P. The results of this study indicate that bacterial isolate and their mutants are good candidates for Glyphosate biodegradation in safe and efficient behavior | ||||
| Keywords | ||||
| wastewater; Glyphosate; bioremediation; 16srDNA; mutation; SDS-PAGE | ||||
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