Metabolic Labeling of Malignant Breast Cells Resistance to Chemotherapy with N-Glycolylneuraminic Acid Allows Differential Surface Detection
AlSadek, D., Badr, H., Ahmed, H. (2022). Metabolic Labeling of Malignant Breast Cells Resistance to Chemotherapy with N-Glycolylneuraminic Acid Allows Differential Surface Detection. EKB Journal Management System, 50(2), 126-135. doi: 10.21608/zvjz.2022.128693.1176
Dina AlSadek; Haitham Badr; Hafiz Ahmed. "Metabolic Labeling of Malignant Breast Cells Resistance to Chemotherapy with N-Glycolylneuraminic Acid Allows Differential Surface Detection". EKB Journal Management System, 50, 2, 2022, 126-135. doi: 10.21608/zvjz.2022.128693.1176
AlSadek, D., Badr, H., Ahmed, H. (2022). 'Metabolic Labeling of Malignant Breast Cells Resistance to Chemotherapy with N-Glycolylneuraminic Acid Allows Differential Surface Detection', EKB Journal Management System, 50(2), pp. 126-135. doi: 10.21608/zvjz.2022.128693.1176
AlSadek, D., Badr, H., Ahmed, H. Metabolic Labeling of Malignant Breast Cells Resistance to Chemotherapy with N-Glycolylneuraminic Acid Allows Differential Surface Detection. EKB Journal Management System, 2022; 50(2): 126-135. doi: 10.21608/zvjz.2022.128693.1176

Metabolic Labeling of Malignant Breast Cells Resistance to Chemotherapy with N-Glycolylneuraminic Acid Allows Differential Surface Detection

Zagazig Veterinary Journal
Article 3, Volume 50, Issue 2, June 2022, Page 126-135  XML PDF (619.1 K)
Document Type: Original Article
DOI: 10.21608/zvjz.2022.128693.1176
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Authors
Dina AlSadek email orcid 1; Haitham Badrorcid 2; Hafiz Ahmedorcid 3
1Department of Histology and Cytology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt
2Department of Biochemistry, Faculty of Agriculture, Zagazig University, Zagazig 44511, Egypt
3GlycoMantra, Inc., Baltimore, MD 21227, USA.
Abstract
The ultimate goal of cancer science is to learn how to select a cancer cell surface. Glycans in cancer cells are frequently at varying quantities or have fundamentally various structures than those seen in normal cells. The presence of sialic acid (Neu5Ac), which is prevalent at the end of glycan chains, is significant. N-glycolylneuraminic acid (Neu5Gc) is a “non-human” sugar type that intercept into Neu5Ac natural biosynthetic machinery, presaging as a perspective biomarker. Malignant breast cells resistance to chemotherapy and their non-malignant counterparts, when treated with the Neu5Gc under nutrient deprivation, display cell surface tumor-associated Neu5Gc-rich glycans, which is capture with Neu5Gc linkage specific plant lectins such as Triticum vulgaris agglutinin (WGA), Sambucus nigra agglutinin (SNA), and Maackia amurensis agglutinin I (MAL-I). Nevertheless, MAL-I binding differentiates surface of malignant breast cell line resistance to chemotherapy MCF-7/H compared to normal mammary epithelial cell line MCF-10/A. These findings emphasize the importance of one oxygen atom in Neu5Gc; it’s supplementing of nutrient-depleted cancer cells' resistance to chemotherapy and facilitates the way for the implementation of better diagnostic and prognostic approaches.
Keywords
oxygen atom; neuraminic acids; glycan metabolism; plant lectins; nutrient deprivation
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