Detection of rmpA and magA genes in Hypervirulent Klebsiella pneumoniae Isolates from Tertiary Care Hospitals
Khattab, M., Hager, R. (2022). Detection of rmpA and magA genes in Hypervirulent Klebsiella pneumoniae Isolates from Tertiary Care Hospitals. EKB Journal Management System, 31(3), 43-50. doi: 10.21608/ejmm.2022.247186
Mona Adel Khattab; Raghda Hager. "Detection of rmpA and magA genes in Hypervirulent Klebsiella pneumoniae Isolates from Tertiary Care Hospitals". EKB Journal Management System, 31, 3, 2022, 43-50. doi: 10.21608/ejmm.2022.247186
Khattab, M., Hager, R. (2022). 'Detection of rmpA and magA genes in Hypervirulent Klebsiella pneumoniae Isolates from Tertiary Care Hospitals', EKB Journal Management System, 31(3), pp. 43-50. doi: 10.21608/ejmm.2022.247186
Khattab, M., Hager, R. Detection of rmpA and magA genes in Hypervirulent Klebsiella pneumoniae Isolates from Tertiary Care Hospitals. EKB Journal Management System, 2022; 31(3): 43-50. doi: 10.21608/ejmm.2022.247186

Detection of rmpA and magA genes in Hypervirulent Klebsiella pneumoniae Isolates from Tertiary Care Hospitals

Egyptian Journal of Medical Microbiology
Volume 31, Issue 3, July 2022, Page 43-50  XML PDF (357.26 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2022.247186
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Authors
Mona Adel Khattab email orcid 1; Raghda Hager2
1Medical Microbiology & Immunology Faculty of Medicine Ain Shams University
2Medical Microbiology &Immunology Faculty of Medicine, Misr University for Science and Technology
Abstract
Background: Hypervirulent Klebsiella pneumoniae (hvKp) cause multiple dangerous infections. HvKp can be distinguished from classical K. pneumoniae (cKp) by hyper-mucoviscosity. Presence of multiple genes contribute to hypervirulent phenotype of K. pneumoniae as mucoviscosity-associated gene A (magA) and regulator of mucoid phenotype A (rmpA). Objectives: to detect hvKp frequency among strains of k.pneumoniae by using string test phenotypically, to use multiplex polymerase chain reaction for rmpA and magA genes detection. Methodology: Fifty isolates of k.pneumoniae, were obtained from hospitalized patients in El-zohour Hospital. Conventional identification and susceptibility to antimicrobials were performed. Results: Most strains were isolated from samples of urine. Thirty strains out of fifty (60%) were hvKp by using string test. magA and rmpA were present in 14 isolates (28%) and 29 isolates (58.0%) respectively among k.pneumoniae isolates. Out of thirty hvKp strains, magA and rmpA genes were present in 14 stains (46.7%) and 25 strains (83.3%) respectively and 12 strains (40%) were coharboring both genes and were string test positive. Conclusions: high frequency of hvKp among clinical samples. Prompt detection of hvKp in different clinical settings is needed through establishing effective diagnostic tools. String test is a sensitive tool for rmpA gene detection. Establishment of both proper preventive measures and antimicrobial stewardship programs may be helpful to overcome spreading of multidrug resistant hvKp.
Keywords
Hypervirulent k. pneumonia (hvKP); string test; mucoviscosity-associated gene A (magA) and regulator of mucoid phenotype A (rmpA)
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